Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20885 and BPHYT_RS20890 are separated by 94 nucleotides BPHYT_RS20890 and BPHYT_RS20895 are separated by 345 nucleotides BPHYT_RS20895 and BPHYT_RS20900 are separated by 319 nucleotides
BPHYT_RS20885: BPHYT_RS20885 - nuclear protein SET, at 246,679 to 247,119
_RS20885
BPHYT_RS20890: BPHYT_RS20890 - transglycosylase, at 247,214 to 247,465
_RS20890
BPHYT_RS20895: BPHYT_RS20895 - ABC transporter, at 247,811 to 249,604
_RS20895
BPHYT_RS20900: BPHYT_RS20900 - phytanoyl-CoA dioxygenase, at 249,924 to 251,138
_RS20900
Position (kb) 247
248
249
250 Strain fitness (log2 ratio) -2
-1
0
1
2 at 247.005 kb on + strand, within BPHYT_RS20885 at 247.013 kb on - strand, within BPHYT_RS20885 at 247.060 kb on + strand, within BPHYT_RS20885 at 247.122 kb on + strand at 247.271 kb on - strand, within BPHYT_RS20890 at 247.372 kb on + strand, within BPHYT_RS20890 at 247.575 kb on + strand at 247.575 kb on + strand at 247.575 kb on + strand at 247.576 kb on - strand at 247.664 kb on - strand at 247.787 kb on + strand at 247.970 kb on + strand at 248.035 kb on - strand, within BPHYT_RS20895 at 248.193 kb on + strand, within BPHYT_RS20895 at 248.194 kb on - strand, within BPHYT_RS20895 at 248.365 kb on + strand, within BPHYT_RS20895 at 248.366 kb on - strand, within BPHYT_RS20895 at 248.482 kb on + strand, within BPHYT_RS20895 at 248.733 kb on + strand, within BPHYT_RS20895 at 248.733 kb on + strand, within BPHYT_RS20895 at 248.880 kb on - strand, within BPHYT_RS20895 at 249.690 kb on + strand at 249.819 kb on + strand at 249.897 kb on + strand at 249.945 kb on - strand at 250.486 kb on - strand, within BPHYT_RS20900
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 247,005 + BPHYT_RS20885 0.74 +0.5 247,013 - BPHYT_RS20885 0.76 -2.0 247,060 + BPHYT_RS20885 0.86 -0.2 247,122 + +0.3 247,271 - BPHYT_RS20890 0.23 +0.1 247,372 + BPHYT_RS20890 0.63 +0.3 247,575 + +1.7 247,575 + +0.2 247,575 + +0.4 247,576 - -0.1 247,664 - -0.0 247,787 + -0.2 247,970 + +0.3 248,035 - BPHYT_RS20895 0.12 +2.4 248,193 + BPHYT_RS20895 0.21 -0.8 248,194 - BPHYT_RS20895 0.21 +0.2 248,365 + BPHYT_RS20895 0.31 -0.2 248,366 - BPHYT_RS20895 0.31 -0.5 248,482 + BPHYT_RS20895 0.37 -0.2 248,733 + BPHYT_RS20895 0.51 -1.0 248,733 + BPHYT_RS20895 0.51 -0.7 248,880 - BPHYT_RS20895 0.60 +0.4 249,690 + +0.2 249,819 + +1.7 249,897 + -0.6 249,945 - +0.6 250,486 - BPHYT_RS20900 0.46 +0.4
Or see this region's nucleotide sequence