Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20880 and BPHYT_RS20885 are separated by 187 nucleotides BPHYT_RS20885 and BPHYT_RS20890 are separated by 94 nucleotides BPHYT_RS20890 and BPHYT_RS20895 are separated by 345 nucleotides
BPHYT_RS20880: BPHYT_RS20880 - polyketide cyclase, at 245,946 to 246,491
_RS20880
BPHYT_RS20885: BPHYT_RS20885 - nuclear protein SET, at 246,679 to 247,119
_RS20885
BPHYT_RS20890: BPHYT_RS20890 - transglycosylase, at 247,214 to 247,465
_RS20890
BPHYT_RS20895: BPHYT_RS20895 - ABC transporter, at 247,811 to 249,604
_RS20895
Position (kb) 247
248 Strain fitness (log2 ratio) -2
-1
0
1
2 at 246.635 kb on - strand at 246.678 kb on + strand at 246.695 kb on + strand at 247.005 kb on + strand, within BPHYT_RS20885 at 247.013 kb on - strand, within BPHYT_RS20885 at 247.060 kb on + strand, within BPHYT_RS20885 at 247.122 kb on + strand at 247.271 kb on - strand, within BPHYT_RS20890 at 247.372 kb on + strand, within BPHYT_RS20890 at 247.575 kb on + strand at 247.575 kb on + strand at 247.575 kb on + strand at 247.576 kb on - strand at 247.664 kb on - strand at 247.787 kb on + strand at 247.970 kb on + strand at 248.035 kb on - strand, within BPHYT_RS20895 at 248.193 kb on + strand, within BPHYT_RS20895 at 248.194 kb on - strand, within BPHYT_RS20895 at 248.365 kb on + strand, within BPHYT_RS20895 at 248.366 kb on - strand, within BPHYT_RS20895
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 246,635 - -0.1 246,678 + -0.8 246,695 + +0.5 247,005 + BPHYT_RS20885 0.74 +0.5 247,013 - BPHYT_RS20885 0.76 -2.0 247,060 + BPHYT_RS20885 0.86 -0.2 247,122 + +0.3 247,271 - BPHYT_RS20890 0.23 +0.1 247,372 + BPHYT_RS20890 0.63 +0.3 247,575 + +1.7 247,575 + +0.2 247,575 + +0.4 247,576 - -0.1 247,664 - -0.0 247,787 + -0.2 247,970 + +0.3 248,035 - BPHYT_RS20895 0.12 +2.4 248,193 + BPHYT_RS20895 0.21 -0.8 248,194 - BPHYT_RS20895 0.21 +0.2 248,365 + BPHYT_RS20895 0.31 -0.2 248,366 - BPHYT_RS20895 0.31 -0.5
Or see this region's nucleotide sequence