Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19930 and BPHYT_RS19935 are separated by 24 nucleotides BPHYT_RS19935 and BPHYT_RS19940 are separated by 5 nucleotides BPHYT_RS19940 and BPHYT_RS19945 are separated by 470 nucleotides
BPHYT_RS19930: BPHYT_RS19930 - transcriptional regulator, at 17,606 to 18,961
_RS19930
BPHYT_RS19935: BPHYT_RS19935 - protein serine/threonine phosphatase, at 18,986 to 19,756
_RS19935
BPHYT_RS19940: BPHYT_RS19940 - hypothetical protein, at 19,762 to 20,049
_RS19940
BPHYT_RS19945: BPHYT_RS19945 - hypothetical protein, at 20,520 to 21,017
_RS19945
Position (kb) 18
19
20 Strain fitness (log2 ratio) -2
-1
0
1 at 18.533 kb on + strand, within BPHYT_RS19930 at 18.533 kb on + strand, within BPHYT_RS19930 at 18.706 kb on - strand, within BPHYT_RS19930 at 19.413 kb on + strand, within BPHYT_RS19935 at 19.469 kb on + strand, within BPHYT_RS19935 at 19.770 kb on + strand at 19.831 kb on + strand, within BPHYT_RS19940 at 19.832 kb on - strand, within BPHYT_RS19940 at 19.871 kb on + strand, within BPHYT_RS19940 at 19.934 kb on + strand, within BPHYT_RS19940 at 19.998 kb on + strand, within BPHYT_RS19940 at 20.040 kb on + strand at 20.041 kb on - strand at 20.106 kb on + strand at 20.107 kb on - strand at 20.120 kb on - strand at 20.120 kb on - strand at 20.643 kb on + strand, within BPHYT_RS19945 at 20.670 kb on + strand, within BPHYT_RS19945
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 18,533 + BPHYT_RS19930 0.68 +0.4 18,533 + BPHYT_RS19930 0.68 -2.7 18,706 - BPHYT_RS19930 0.81 -1.0 19,413 + BPHYT_RS19935 0.55 +0.7 19,469 + BPHYT_RS19935 0.63 -0.0 19,770 + -1.2 19,831 + BPHYT_RS19940 0.24 -1.2 19,832 - BPHYT_RS19940 0.24 +0.7 19,871 + BPHYT_RS19940 0.38 -1.2 19,934 + BPHYT_RS19940 0.60 +0.2 19,998 + BPHYT_RS19940 0.82 -0.5 20,040 + -0.9 20,041 - -1.4 20,106 + -0.5 20,107 - -0.1 20,120 - +0.7 20,120 - +1.0 20,643 + BPHYT_RS19945 0.25 -1.0 20,670 + BPHYT_RS19945 0.30 -0.9
Or see this region's nucleotide sequence