Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19880 and BPHYT_RS19885 are separated by 72 nucleotides BPHYT_RS19885 and BPHYT_RS19890 are separated by 368 nucleotides BPHYT_RS19890 and BPHYT_RS19895 are separated by 47 nucleotides
BPHYT_RS19880: BPHYT_RS19880 - protein kinase, at 1,730 to 3,865
_RS19880
BPHYT_RS19885: BPHYT_RS19885 - hypothetical protein, at 3,938 to 5,911
_RS19885
BPHYT_RS19890: BPHYT_RS19890 - hypothetical protein, at 6,280 to 6,783
_RS19890
BPHYT_RS19895: BPHYT_RS19895 - rod shape-determining protein RodA, at 6,831 to 7,265
_RS19895
Position (kb) 3
4
5
6 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3.016 kb on + strand, within BPHYT_RS19880 at 3.527 kb on + strand, within BPHYT_RS19880 at 3.536 kb on + strand, within BPHYT_RS19880 at 3.554 kb on + strand, within BPHYT_RS19880 at 3.759 kb on - strand at 3.817 kb on + strand at 3.817 kb on + strand at 4.287 kb on + strand, within BPHYT_RS19885 at 4.510 kb on - strand, within BPHYT_RS19885 at 4.917 kb on + strand, within BPHYT_RS19885 at 5.202 kb on + strand, within BPHYT_RS19885 at 5.266 kb on + strand, within BPHYT_RS19885 at 5.330 kb on - strand, within BPHYT_RS19885 at 5.943 kb on - strand at 6.035 kb on - strand at 6.038 kb on - strand at 6.112 kb on + strand at 6.113 kb on - strand at 6.128 kb on + strand at 6.129 kb on - strand at 6.129 kb on - strand at 6.129 kb on - strand at 6.379 kb on - strand, within BPHYT_RS19890
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 3,016 + BPHYT_RS19880 0.60 +1.1 3,527 + BPHYT_RS19880 0.84 +0.7 3,536 + BPHYT_RS19880 0.85 -0.1 3,554 + BPHYT_RS19880 0.85 -2.2 3,759 - +0.4 3,817 + -0.0 3,817 + -0.7 4,287 + BPHYT_RS19885 0.18 -0.0 4,510 - BPHYT_RS19885 0.29 +0.3 4,917 + BPHYT_RS19885 0.50 +0.4 5,202 + BPHYT_RS19885 0.64 +0.0 5,266 + BPHYT_RS19885 0.67 +1.5 5,330 - BPHYT_RS19885 0.71 +0.4 5,943 - +0.5 6,035 - +2.0 6,038 - +0.4 6,112 + -0.5 6,113 - -1.0 6,128 + -1.0 6,129 - -0.5 6,129 - -1.9 6,129 - -2.3 6,379 - BPHYT_RS19890 0.20 +0.5
Or see this region's nucleotide sequence