Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14885 and BPHYT_RS14890 are separated by 165 nucleotides BPHYT_RS14890 and BPHYT_RS14895 are separated by 35 nucleotides BPHYT_RS14895 and BPHYT_RS14900 are separated by 19 nucleotides
BPHYT_RS14885: BPHYT_RS14885 - 30S ribosomal protein S1, at 3,384,766 to 3,386,499
_RS14885
BPHYT_RS14890: BPHYT_RS14890 - cytidylate kinase, at 3,386,665 to 3,387,351
_RS14890
BPHYT_RS14895: BPHYT_RS14895 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 3,387,387 to 3,388,691
_RS14895
BPHYT_RS14900: BPHYT_RS14900 - prephenate dehydrogenase, at 3,388,711 to 3,389,652
_RS14900
Position (kb)
3387
3388
3389 Strain fitness (log2 ratio)
-1
0
1
2 at 3386.681 kb on - strand at 3387.450 kb on - strand at 3387.524 kb on - strand, within BPHYT_RS14895 at 3387.578 kb on - strand, within BPHYT_RS14895 at 3387.578 kb on - strand, within BPHYT_RS14895 at 3387.672 kb on - strand, within BPHYT_RS14895 at 3388.364 kb on - strand, within BPHYT_RS14895 at 3389.193 kb on - strand, within BPHYT_RS14900 at 3389.626 kb on - strand at 3389.657 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 3,386,681 - -0.3 3,387,450 - -0.8 3,387,524 - BPHYT_RS14895 0.10 -0.3 3,387,578 - BPHYT_RS14895 0.15 +2.3 3,387,578 - BPHYT_RS14895 0.15 -0.3 3,387,672 - BPHYT_RS14895 0.22 -1.3 3,388,364 - BPHYT_RS14895 0.75 +0.3 3,389,193 - BPHYT_RS14900 0.51 -0.1 3,389,626 - -0.5 3,389,657 - -0.3
Or see this region's nucleotide sequence