Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14270 and BPHYT_RS14275 are separated by 18 nucleotides BPHYT_RS14275 and BPHYT_RS14280 are separated by 17 nucleotides BPHYT_RS14280 and BPHYT_RS14285 are separated by 60 nucleotides BPHYT_RS14285 and BPHYT_RS14290 are separated by 88 nucleotides
BPHYT_RS14270: BPHYT_RS14270 - electron transporter, at 3,261,905 to 3,262,519
_RS14270
BPHYT_RS14275: BPHYT_RS14275 - hypothetical protein, at 3,262,538 to 3,262,999
_RS14275
BPHYT_RS14280: BPHYT_RS14280 - membrane protein, at 3,263,017 to 3,263,886
_RS14280
BPHYT_RS14285: BPHYT_RS14285 - drug:proton antiporter, at 3,263,947 to 3,264,441
_RS14285
BPHYT_RS14290: BPHYT_RS14290 - diguanylate cyclase, at 3,264,530 to 3,265,510
_RS14290
Position (kb)
3263
3264 Strain fitness (log2 ratio)
-1
0
1
2 at 3262.113 kb on - strand, within BPHYT_RS14270 at 3262.122 kb on - strand, within BPHYT_RS14270 at 3262.265 kb on + strand, within BPHYT_RS14270 at 3262.265 kb on + strand, within BPHYT_RS14270 at 3262.265 kb on + strand, within BPHYT_RS14270 at 3262.266 kb on - strand, within BPHYT_RS14270 at 3262.266 kb on - strand, within BPHYT_RS14270 at 3262.388 kb on + strand, within BPHYT_RS14270 at 3262.389 kb on - strand, within BPHYT_RS14270 at 3262.402 kb on + strand, within BPHYT_RS14270 at 3262.402 kb on + strand, within BPHYT_RS14270 at 3262.402 kb on + strand, within BPHYT_RS14270 at 3262.415 kb on + strand, within BPHYT_RS14270 at 3262.416 kb on - strand, within BPHYT_RS14270 at 3262.755 kb on - strand, within BPHYT_RS14275 at 3262.997 kb on + strand at 3262.998 kb on - strand at 3263.029 kb on + strand at 3263.171 kb on - strand, within BPHYT_RS14280 at 3263.171 kb on - strand, within BPHYT_RS14280 at 3263.171 kb on - strand, within BPHYT_RS14280 at 3263.171 kb on - strand, within BPHYT_RS14280 at 3263.198 kb on - strand, within BPHYT_RS14280 at 3263.316 kb on + strand, within BPHYT_RS14280 at 3263.317 kb on - strand, within BPHYT_RS14280 at 3263.428 kb on + strand, within BPHYT_RS14280 at 3263.672 kb on - strand, within BPHYT_RS14280 at 3263.829 kb on + strand at 3263.890 kb on + strand at 3264.248 kb on + strand, within BPHYT_RS14285 at 3264.249 kb on - strand, within BPHYT_RS14285 at 3264.297 kb on - strand, within BPHYT_RS14285 at 3264.348 kb on + strand, within BPHYT_RS14285 at 3264.348 kb on + strand, within BPHYT_RS14285 at 3264.348 kb on + strand, within BPHYT_RS14285 at 3264.348 kb on + strand, within BPHYT_RS14285 at 3264.348 kb on + strand, within BPHYT_RS14285 at 3264.349 kb on - strand, within BPHYT_RS14285 at 3264.349 kb on - strand, within BPHYT_RS14285 at 3264.349 kb on - strand, within BPHYT_RS14285 at 3264.349 kb on - strand, within BPHYT_RS14285 at 3264.349 kb on - strand, within BPHYT_RS14285 at 3264.350 kb on + strand, within BPHYT_RS14285 at 3264.350 kb on + strand, within BPHYT_RS14285 at 3264.351 kb on - strand, within BPHYT_RS14285 at 3264.452 kb on + strand at 3264.494 kb on + strand at 3264.576 kb on + strand at 3264.576 kb on + strand at 3264.577 kb on - strand at 3264.577 kb on - strand at 3264.636 kb on + strand, within BPHYT_RS14290 at 3264.637 kb on - strand, within BPHYT_RS14290 at 3264.637 kb on - strand, within BPHYT_RS14290 at 3264.669 kb on + strand, within BPHYT_RS14290
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 3,262,113 - BPHYT_RS14270 0.34 +1.9 3,262,122 - BPHYT_RS14270 0.35 +1.0 3,262,265 + BPHYT_RS14270 0.59 +0.8 3,262,265 + BPHYT_RS14270 0.59 +0.2 3,262,265 + BPHYT_RS14270 0.59 -0.2 3,262,266 - BPHYT_RS14270 0.59 +0.1 3,262,266 - BPHYT_RS14270 0.59 -0.8 3,262,388 + BPHYT_RS14270 0.79 -1.1 3,262,389 - BPHYT_RS14270 0.79 +0.8 3,262,402 + BPHYT_RS14270 0.81 +1.0 3,262,402 + BPHYT_RS14270 0.81 -0.3 3,262,402 + BPHYT_RS14270 0.81 +0.3 3,262,415 + BPHYT_RS14270 0.83 -0.6 3,262,416 - BPHYT_RS14270 0.83 +0.8 3,262,755 - BPHYT_RS14275 0.47 -0.3 3,262,997 + +1.5 3,262,998 - -0.3 3,263,029 + +1.0 3,263,171 - BPHYT_RS14280 0.18 +0.7 3,263,171 - BPHYT_RS14280 0.18 -0.3 3,263,171 - BPHYT_RS14280 0.18 +0.1 3,263,171 - BPHYT_RS14280 0.18 +0.7 3,263,198 - BPHYT_RS14280 0.21 +2.6 3,263,316 + BPHYT_RS14280 0.34 -0.4 3,263,317 - BPHYT_RS14280 0.34 +1.0 3,263,428 + BPHYT_RS14280 0.47 +0.7 3,263,672 - BPHYT_RS14280 0.75 -0.1 3,263,829 + -0.6 3,263,890 + +0.6 3,264,248 + BPHYT_RS14285 0.61 +0.3 3,264,249 - BPHYT_RS14285 0.61 -0.3 3,264,297 - BPHYT_RS14285 0.71 -1.7 3,264,348 + BPHYT_RS14285 0.81 -0.5 3,264,348 + BPHYT_RS14285 0.81 -0.3 3,264,348 + BPHYT_RS14285 0.81 +0.2 3,264,348 + BPHYT_RS14285 0.81 +0.6 3,264,348 + BPHYT_RS14285 0.81 +1.0 3,264,349 - BPHYT_RS14285 0.81 +1.5 3,264,349 - BPHYT_RS14285 0.81 -0.3 3,264,349 - BPHYT_RS14285 0.81 +1.1 3,264,349 - BPHYT_RS14285 0.81 +0.4 3,264,349 - BPHYT_RS14285 0.81 -0.6 3,264,350 + BPHYT_RS14285 0.81 +0.3 3,264,350 + BPHYT_RS14285 0.81 +0.7 3,264,351 - BPHYT_RS14285 0.82 +0.5 3,264,452 + +0.2 3,264,494 + -0.1 3,264,576 + -0.7 3,264,576 + -0.6 3,264,577 - +0.6 3,264,577 - +0.9 3,264,636 + BPHYT_RS14290 0.11 -0.4 3,264,637 - BPHYT_RS14290 0.11 -0.7 3,264,637 - BPHYT_RS14290 0.11 -0.2 3,264,669 + BPHYT_RS14290 0.14 +0.3
Or see this region's nucleotide sequence