Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10790 and BPHYT_RS10795 are separated by 46 nucleotides BPHYT_RS10795 and BPHYT_RS10800 are separated by 176 nucleotides BPHYT_RS10800 and BPHYT_RS10805 overlap by 4 nucleotides
BPHYT_RS10790: BPHYT_RS10790 - C4-dicarboxylate ABC transporter substrate-binding protein, at 2,452,334 to 2,453,686
_RS10790
BPHYT_RS10795: BPHYT_RS10795 - ProQ activator of osmoprotectant transporter ProP, at 2,453,733 to 2,454,488
_RS10795
BPHYT_RS10800: BPHYT_RS10800 - LysR family transcriptional regulator, at 2,454,665 to 2,455,588
_RS10800
BPHYT_RS10805: BPHYT_RS10805 - MFS transporter, at 2,455,585 to 2,456,961
_RS10805
Position (kb)
2454
2455
2456 Strain fitness (log2 ratio)
-2
-1
0
1 at 2453.704 kb on - strand at 2454.521 kb on - strand at 2454.581 kb on + strand at 2454.743 kb on - strand at 2454.857 kb on + strand at 2454.866 kb on + strand, within BPHYT_RS10800 at 2454.866 kb on + strand, within BPHYT_RS10800 at 2454.867 kb on - strand, within BPHYT_RS10800 at 2454.867 kb on - strand, within BPHYT_RS10800 at 2455.183 kb on - strand, within BPHYT_RS10800 at 2455.586 kb on + strand at 2455.899 kb on + strand, within BPHYT_RS10805 at 2455.899 kb on + strand, within BPHYT_RS10805 at 2456.130 kb on - strand, within BPHYT_RS10805 at 2456.423 kb on + strand, within BPHYT_RS10805 at 2456.496 kb on - strand, within BPHYT_RS10805
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 2,453,704 - +0.0 2,454,521 - +0.2 2,454,581 + -1.8 2,454,743 - -1.4 2,454,857 + +0.7 2,454,866 + BPHYT_RS10800 0.22 +0.5 2,454,866 + BPHYT_RS10800 0.22 +0.2 2,454,867 - BPHYT_RS10800 0.22 -0.5 2,454,867 - BPHYT_RS10800 0.22 -1.4 2,455,183 - BPHYT_RS10800 0.56 +0.9 2,455,586 + -0.3 2,455,899 + BPHYT_RS10805 0.23 -1.6 2,455,899 + BPHYT_RS10805 0.23 -1.0 2,456,130 - BPHYT_RS10805 0.40 +0.1 2,456,423 + BPHYT_RS10805 0.61 -1.4 2,456,496 - BPHYT_RS10805 0.66 -0.6
Or see this region's nucleotide sequence