Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07215 and BPHYT_RS07220 are separated by 105 nucleotides BPHYT_RS07220 and BPHYT_RS07225 are separated by 211 nucleotides BPHYT_RS07225 and BPHYT_RS07230 are separated by 202 nucleotides
BPHYT_RS07215: BPHYT_RS07215 - LysR family transcriptional regulator, at 1,639,849 to 1,640,754
_RS07215
BPHYT_RS07220: BPHYT_RS07220 - GCN5 family acetyltransferase, at 1,640,860 to 1,641,489
_RS07220
BPHYT_RS07225: BPHYT_RS07225 - membrane protein, at 1,641,701 to 1,641,862
_RS07225
BPHYT_RS07230: BPHYT_RS07230 - LysR family transcriptional regulator, at 1,642,065 to 1,643,042
_RS07230
Position (kb) 1641
1642 Strain fitness (log2 ratio) -1
0
1 at 1640.895 kb on + strand at 1640.902 kb on + strand at 1640.903 kb on - strand at 1641.161 kb on + strand, within BPHYT_RS07220 at 1641.162 kb on - strand, within BPHYT_RS07220 at 1641.891 kb on + strand at 1641.966 kb on - strand at 1642.141 kb on + strand at 1642.338 kb on - strand, within BPHYT_RS07230 at 1642.779 kb on - strand, within BPHYT_RS07230 at 1642.826 kb on + strand, within BPHYT_RS07230
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 1,640,895 + -0.6 1,640,902 + +0.1 1,640,903 - -0.9 1,641,161 + BPHYT_RS07220 0.48 -1.4 1,641,162 - BPHYT_RS07220 0.48 -1.2 1,641,891 + -1.0 1,641,966 - -0.0 1,642,141 + -0.9 1,642,338 - BPHYT_RS07230 0.28 -1.6 1,642,779 - BPHYT_RS07230 0.73 +0.3 1,642,826 + BPHYT_RS07230 0.78 +1.2
Or see this region's nucleotide sequence