Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07040 and BPHYT_RS07045 are separated by 264 nucleotides BPHYT_RS07045 and BPHYT_RS07050 are separated by 413 nucleotides
BPHYT_RS07040: BPHYT_RS07040 - asparaginase, at 1,591,636 to 1,592,727
_RS07040
BPHYT_RS07045: BPHYT_RS07045 - chemotaxis protein CheV, at 1,592,992 to 1,593,969
_RS07045
BPHYT_RS07050: BPHYT_RS07050 - membrane protein, at 1,594,383 to 1,594,943
_RS07050
Position (kb)
1592
1593
1594 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1591.997 kb on - strand, within BPHYT_RS07040 at 1592.342 kb on - strand, within BPHYT_RS07040 at 1592.360 kb on - strand, within BPHYT_RS07040 at 1592.558 kb on - strand, within BPHYT_RS07040 at 1592.820 kb on + strand at 1593.074 kb on + strand at 1593.075 kb on - strand at 1593.138 kb on + strand, within BPHYT_RS07045 at 1593.175 kb on - strand, within BPHYT_RS07045 at 1593.332 kb on + strand, within BPHYT_RS07045 at 1593.346 kb on - strand, within BPHYT_RS07045 at 1593.460 kb on - strand, within BPHYT_RS07045 at 1593.500 kb on - strand, within BPHYT_RS07045 at 1593.500 kb on - strand, within BPHYT_RS07045 at 1593.545 kb on - strand, within BPHYT_RS07045 at 1594.309 kb on + strand at 1594.309 kb on + strand at 1594.382 kb on + strand at 1594.383 kb on - strand at 1594.383 kb on - strand at 1594.383 kb on - strand at 1594.761 kb on + strand, within BPHYT_RS07050 at 1594.762 kb on - strand, within BPHYT_RS07050 at 1594.890 kb on + strand at 1594.891 kb on - strand at 1594.919 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 1,591,997 - BPHYT_RS07040 0.33 -1.3 1,592,342 - BPHYT_RS07040 0.65 -0.3 1,592,360 - BPHYT_RS07040 0.66 +0.7 1,592,558 - BPHYT_RS07040 0.84 +0.5 1,592,820 + +2.9 1,593,074 + +1.3 1,593,075 - -0.0 1,593,138 + BPHYT_RS07045 0.15 -0.5 1,593,175 - BPHYT_RS07045 0.19 -1.5 1,593,332 + BPHYT_RS07045 0.35 +1.0 1,593,346 - BPHYT_RS07045 0.36 +1.1 1,593,460 - BPHYT_RS07045 0.48 -3.5 1,593,500 - BPHYT_RS07045 0.52 +0.4 1,593,500 - BPHYT_RS07045 0.52 -1.5 1,593,545 - BPHYT_RS07045 0.57 -0.7 1,594,309 + -1.0 1,594,309 + -0.0 1,594,382 + +0.6 1,594,383 - +2.3 1,594,383 - +0.2 1,594,383 - -0.5 1,594,761 + BPHYT_RS07050 0.67 -0.0 1,594,762 - BPHYT_RS07050 0.68 -1.6 1,594,890 + -0.6 1,594,891 - -0.0 1,594,919 + -0.4
Or see this region's nucleotide sequence