Experiment: 24hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01770 and BPHYT_RS01775 are separated by 378 nucleotides BPHYT_RS01775 and BPHYT_RS01780 overlap by 4 nucleotides BPHYT_RS01780 and BPHYT_RS01785 are separated by 375 nucleotides
BPHYT_RS01770: BPHYT_RS01770 - carbon monoxide dehydrogenase, at 403,572 to 404,090
_RS01770
BPHYT_RS01775: BPHYT_RS01775 - hypothetical protein, at 404,469 to 404,813
_RS01775
BPHYT_RS01780: BPHYT_RS01780 - hypothetical protein, at 404,810 to 405,178
_RS01780
BPHYT_RS01785: BPHYT_RS01785 - amino acid permease, at 405,554 to 406,945
_RS01785
Position (kb) 404
405
406 Strain fitness (log2 ratio) -2
-1
0
1 at 404.202 kb on - strand at 404.226 kb on + strand at 404.305 kb on - strand at 404.307 kb on + strand at 404.307 kb on + strand at 404.308 kb on - strand at 404.335 kb on - strand at 404.406 kb on + strand at 404.425 kb on + strand at 404.425 kb on + strand at 404.425 kb on + strand at 404.425 kb on + strand at 404.426 kb on - strand at 404.470 kb on + strand at 404.588 kb on + strand, within BPHYT_RS01775 at 404.589 kb on - strand, within BPHYT_RS01775 at 404.596 kb on + strand, within BPHYT_RS01775 at 404.597 kb on - strand, within BPHYT_RS01775 at 404.705 kb on - strand, within BPHYT_RS01775 at 404.717 kb on + strand, within BPHYT_RS01775 at 404.718 kb on - strand, within BPHYT_RS01775 at 404.720 kb on + strand, within BPHYT_RS01775 at 404.721 kb on - strand, within BPHYT_RS01775 at 404.721 kb on - strand, within BPHYT_RS01775 at 404.820 kb on - strand at 404.867 kb on + strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.868 kb on - strand, within BPHYT_RS01780 at 404.876 kb on - strand, within BPHYT_RS01780 at 405.024 kb on - strand, within BPHYT_RS01780 at 405.144 kb on - strand at 405.146 kb on - strand at 405.214 kb on + strand at 405.214 kb on + strand at 405.284 kb on - strand at 405.325 kb on - strand at 405.373 kb on + strand at 405.373 kb on + strand at 405.527 kb on + strand at 405.797 kb on - strand, within BPHYT_RS01785 at 405.880 kb on + strand at 405.986 kb on + strand, within BPHYT_RS01785 at 405.987 kb on - strand, within BPHYT_RS01785 at 405.987 kb on - strand, within BPHYT_RS01785 at 405.987 kb on - strand, within BPHYT_RS01785 at 406.069 kb on - strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.143 kb on + strand, within BPHYT_RS01785 at 406.144 kb on - strand, within BPHYT_RS01785 at 406.144 kb on - strand, within BPHYT_RS01785
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 1 remove 404,202 - -0.8 404,226 + +0.2 404,305 - +0.6 404,307 + -1.1 404,307 + +0.5 404,308 - +0.2 404,335 - +0.0 404,406 + -1.2 404,425 + +1.1 404,425 + -2.6 404,425 + -0.8 404,425 + -0.8 404,426 - +0.9 404,470 + -0.0 404,588 + BPHYT_RS01775 0.34 -0.2 404,589 - BPHYT_RS01775 0.35 +0.4 404,596 + BPHYT_RS01775 0.37 +0.0 404,597 - BPHYT_RS01775 0.37 -1.0 404,705 - BPHYT_RS01775 0.68 -1.1 404,717 + BPHYT_RS01775 0.72 +0.8 404,718 - BPHYT_RS01775 0.72 -1.0 404,720 + BPHYT_RS01775 0.73 +0.2 404,721 - BPHYT_RS01775 0.73 -1.9 404,721 - BPHYT_RS01775 0.73 -0.3 404,820 - -0.2 404,867 + BPHYT_RS01780 0.15 -0.4 404,868 - BPHYT_RS01780 0.16 -0.8 404,868 - BPHYT_RS01780 0.16 +0.8 404,868 - BPHYT_RS01780 0.16 -0.5 404,876 - BPHYT_RS01780 0.18 -0.9 405,024 - BPHYT_RS01780 0.58 -1.8 405,144 - -0.0 405,146 - +0.7 405,214 + -0.6 405,214 + +0.7 405,284 - -0.4 405,325 - +0.3 405,373 + -0.3 405,373 + -0.1 405,527 + -1.2 405,797 - BPHYT_RS01785 0.17 +0.3 405,880 + -0.5 405,986 + BPHYT_RS01785 0.31 -0.2 405,987 - BPHYT_RS01785 0.31 +0.1 405,987 - BPHYT_RS01785 0.31 -0.6 405,987 - BPHYT_RS01785 0.31 -0.1 406,069 - BPHYT_RS01785 0.37 +0.1 406,143 + BPHYT_RS01785 0.42 +0.0 406,143 + BPHYT_RS01785 0.42 -1.6 406,143 + BPHYT_RS01785 0.42 -1.1 406,143 + BPHYT_RS01785 0.42 +0.3 406,143 + BPHYT_RS01785 0.42 -0.1 406,144 - BPHYT_RS01785 0.42 +1.0 406,144 - BPHYT_RS01785 0.42 -0.0
Or see this region's nucleotide sequence