Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36470 and BPHYT_RS36475 are separated by 431 nucleotides BPHYT_RS36475 and BPHYT_RS36480 are separated by 124 nucleotides BPHYT_RS36480 and BPHYT_RS36485 are separated by 65 nucleotides
BPHYT_RS36470: BPHYT_RS36470 - hypothetical protein, at 103,926 to 104,846
_RS36470
BPHYT_RS36475: BPHYT_RS36475 - hypothetical protein, at 105,278 to 105,775
_RS36475
BPHYT_RS36480: BPHYT_RS36480 - hypothetical protein, at 105,900 to 106,565
_RS36480
BPHYT_RS36485: BPHYT_RS36485 - hypothetical protein, at 106,631 to 107,155
_RS36485
Position (kb) 105
106 Strain fitness (log2 ratio) -2
-1
0
1 at 104.986 kb on + strand at 105.113 kb on - strand at 105.210 kb on + strand at 105.213 kb on + strand at 105.279 kb on - strand at 105.368 kb on - strand, within BPHYT_RS36475 at 105.462 kb on - strand, within BPHYT_RS36475 at 105.617 kb on + strand, within BPHYT_RS36475 at 105.618 kb on - strand, within BPHYT_RS36475 at 106.166 kb on + strand, within BPHYT_RS36480 at 106.167 kb on - strand, within BPHYT_RS36480 at 106.248 kb on - strand at 106.261 kb on - strand, within BPHYT_RS36480 at 106.442 kb on - strand, within BPHYT_RS36480 at 106.551 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 104,986 + -0.1 105,113 - -1.6 105,210 + -1.6 105,213 + -0.5 105,279 - +0.6 105,368 - BPHYT_RS36475 0.18 +0.5 105,462 - BPHYT_RS36475 0.37 +0.8 105,617 + BPHYT_RS36475 0.68 +0.3 105,618 - BPHYT_RS36475 0.68 +0.0 106,166 + BPHYT_RS36480 0.40 +0.5 106,167 - BPHYT_RS36480 0.40 +0.9 106,248 - +0.5 106,261 - BPHYT_RS36480 0.54 -2.6 106,442 - BPHYT_RS36480 0.81 +0.0 106,551 - +0.2
Or see this region's nucleotide sequence