Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS36320

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS36315 and BPHYT_RS36320 are separated by 2 nucleotidesBPHYT_RS36320 and BPHYT_RS36325 are separated by 77 nucleotides BPHYT_RS36315: BPHYT_RS36315 - CagE TrbE VirB component of type IV transporter system, at 74,136 to 76,685 _RS36315 BPHYT_RS36320: BPHYT_RS36320 - type IV secretion system family protein, at 76,688 to 77,392 _RS36320 BPHYT_RS36325: BPHYT_RS36325 - TrbL/VirB6 plasmid conjugal transfer protein, at 77,470 to 78,468 _RS36325 Position (kb) 76 77 78Strain fitness (log2 ratio) -2 -1 0 1at 75.738 kb on + strand, within BPHYT_RS36315at 75.781 kb on - strand, within BPHYT_RS36315at 75.915 kb on - strand, within BPHYT_RS36315at 76.054 kb on - strand, within BPHYT_RS36315at 76.173 kb on - strand, within BPHYT_RS36315at 76.182 kb on - strand, within BPHYT_RS36315at 76.182 kb on - strand, within BPHYT_RS36315at 76.326 kb on - strand, within BPHYT_RS36315at 76.432 kb on - strandat 76.535 kb on - strandat 77.127 kb on - strand, within BPHYT_RS36320at 77.127 kb on - strand, within BPHYT_RS36320at 77.145 kb on - strand, within BPHYT_RS36320at 77.713 kb on + strand, within BPHYT_RS36325at 77.775 kb on + strand, within BPHYT_RS36325at 77.805 kb on + strand, within BPHYT_RS36325at 77.806 kb on - strand, within BPHYT_RS36325at 78.022 kb on - strand, within BPHYT_RS36325at 78.083 kb on - strand, within BPHYT_RS36325at 78.085 kb on + strand, within BPHYT_RS36325at 78.086 kb on - strand, within BPHYT_RS36325at 78.137 kb on + strand, within BPHYT_RS36325at 78.148 kb on + strand, within BPHYT_RS36325at 78.148 kb on + strand, within BPHYT_RS36325at 78.283 kb on + strand, within BPHYT_RS36325at 78.283 kb on + strand, within BPHYT_RS36325

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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75,738 + BPHYT_RS36315 0.63 -0.1
75,781 - BPHYT_RS36315 0.65 -0.7
75,915 - BPHYT_RS36315 0.70 +0.3
76,054 - BPHYT_RS36315 0.75 -0.5
76,173 - BPHYT_RS36315 0.80 +1.6
76,182 - BPHYT_RS36315 0.80 +0.1
76,182 - BPHYT_RS36315 0.80 +0.1
76,326 - BPHYT_RS36315 0.86 -0.1
76,432 - +1.1
76,535 - +0.3
77,127 - BPHYT_RS36320 0.62 +0.3
77,127 - BPHYT_RS36320 0.62 +0.9
77,145 - BPHYT_RS36320 0.65 +1.2
77,713 + BPHYT_RS36325 0.24 -1.3
77,775 + BPHYT_RS36325 0.31 +0.1
77,805 + BPHYT_RS36325 0.34 +0.2
77,806 - BPHYT_RS36325 0.34 -0.6
78,022 - BPHYT_RS36325 0.55 -0.7
78,083 - BPHYT_RS36325 0.61 +0.7
78,085 + BPHYT_RS36325 0.62 +1.0
78,086 - BPHYT_RS36325 0.62 -2.4
78,137 + BPHYT_RS36325 0.67 -1.6
78,148 + BPHYT_RS36325 0.68 +0.9
78,148 + BPHYT_RS36325 0.68 -0.6
78,283 + BPHYT_RS36325 0.81 +0.5
78,283 + BPHYT_RS36325 0.81 -1.7

Or see this region's nucleotide sequence