Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35390 and BPHYT_RS35395 are separated by 14 nucleotides BPHYT_RS35395 and BPHYT_RS35400 are separated by 4 nucleotides BPHYT_RS35400 and BPHYT_RS35405 are separated by 404 nucleotides BPHYT_RS35405 and BPHYT_RS35410 are separated by 155 nucleotides
BPHYT_RS35390: BPHYT_RS35390 - MFS transporter, at 3,539,423 to 3,540,727
_RS35390
BPHYT_RS35395: BPHYT_RS35395 - membrane protein, at 3,540,742 to 3,541,611
_RS35395
BPHYT_RS35400: BPHYT_RS35400 - ABC transporter, at 3,541,616 to 3,543,280
_RS35400
BPHYT_RS35405: BPHYT_RS35405 - hypothetical protein, at 3,543,685 to 3,543,912
_RS35405
BPHYT_RS35410: BPHYT_RS35410 - alpha/beta hydrolase, at 3,544,068 to 3,545,120
_RS35410
Position (kb)
3541
3542
3543
3544 Strain fitness (log2 ratio)
-1
0
1
2 at 3540.934 kb on + strand, within BPHYT_RS35395 at 3540.934 kb on + strand, within BPHYT_RS35395 at 3540.935 kb on - strand, within BPHYT_RS35395 at 3541.327 kb on + strand, within BPHYT_RS35395 at 3541.617 kb on + strand at 3542.135 kb on - strand, within BPHYT_RS35400 at 3542.590 kb on - strand, within BPHYT_RS35400 at 3542.698 kb on - strand, within BPHYT_RS35400 at 3542.864 kb on - strand, within BPHYT_RS35400 at 3542.941 kb on - strand, within BPHYT_RS35400 at 3543.281 kb on - strand at 3543.460 kb on + strand at 3543.506 kb on - strand at 3543.751 kb on - strand, within BPHYT_RS35405 at 3543.777 kb on - strand, within BPHYT_RS35405 at 3543.953 kb on + strand at 3544.009 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 3,540,934 + BPHYT_RS35395 0.22 +1.6 3,540,934 + BPHYT_RS35395 0.22 +0.2 3,540,935 - BPHYT_RS35395 0.22 -0.5 3,541,327 + BPHYT_RS35395 0.67 +0.2 3,541,617 + -0.1 3,542,135 - BPHYT_RS35400 0.31 -0.7 3,542,590 - BPHYT_RS35400 0.58 -1.5 3,542,698 - BPHYT_RS35400 0.65 +0.1 3,542,864 - BPHYT_RS35400 0.75 -1.4 3,542,941 - BPHYT_RS35400 0.80 +0.4 3,543,281 - -0.3 3,543,460 + +2.6 3,543,506 - -0.7 3,543,751 - BPHYT_RS35405 0.29 -1.6 3,543,777 - BPHYT_RS35405 0.40 -0.3 3,543,953 + -0.5 3,544,009 - -1.0
Or see this region's nucleotide sequence