Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33220 and BPHYT_RS33225 are separated by 472 nucleotides
BPHYT_RS33220: BPHYT_RS33220 - group 1 glycosyl transferase, at 3,030,646 to 3,032,643
_RS33220
BPHYT_RS33225: BPHYT_RS33225 - NAD-dependent epimerase/dehydratase, at 3,033,116 to 3,034,060
_RS33225
Position (kb)
3033
3034
3035 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3032.117 kb on + strand, within BPHYT_RS33220 at 3032.129 kb on + strand, within BPHYT_RS33220 at 3032.130 kb on - strand, within BPHYT_RS33220 at 3032.133 kb on - strand, within BPHYT_RS33220 at 3032.133 kb on - strand, within BPHYT_RS33220 at 3032.269 kb on - strand, within BPHYT_RS33220 at 3032.628 kb on + strand at 3032.664 kb on + strand at 3032.720 kb on - strand at 3033.075 kb on + strand at 3033.151 kb on - strand at 3033.151 kb on - strand at 3033.332 kb on + strand, within BPHYT_RS33225 at 3033.575 kb on - strand, within BPHYT_RS33225 at 3033.606 kb on + strand, within BPHYT_RS33225 at 3033.614 kb on + strand, within BPHYT_RS33225 at 3033.619 kb on - strand, within BPHYT_RS33225 at 3033.656 kb on + strand, within BPHYT_RS33225 at 3033.657 kb on - strand, within BPHYT_RS33225 at 3033.773 kb on + strand, within BPHYT_RS33225 at 3033.796 kb on + strand, within BPHYT_RS33225 at 3034.065 kb on + strand at 3034.111 kb on - strand at 3034.127 kb on - strand at 3034.194 kb on - strand at 3034.225 kb on - strand at 3034.225 kb on - strand at 3034.258 kb on - strand at 3034.397 kb on - strand at 3034.409 kb on + strand at 3034.457 kb on - strand at 3034.463 kb on + strand at 3034.695 kb on - strand at 3034.756 kb on - strand at 3034.828 kb on - strand at 3034.877 kb on + strand at 3034.878 kb on - strand at 3034.997 kb on + strand at 3034.997 kb on + strand at 3034.998 kb on - strand at 3034.998 kb on - strand at 3034.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 3,032,117 + BPHYT_RS33220 0.74 +0.2 3,032,129 + BPHYT_RS33220 0.74 -0.9 3,032,130 - BPHYT_RS33220 0.74 -1.9 3,032,133 - BPHYT_RS33220 0.74 -1.1 3,032,133 - BPHYT_RS33220 0.74 -0.9 3,032,269 - BPHYT_RS33220 0.81 +0.3 3,032,628 + +0.5 3,032,664 + +2.1 3,032,720 - +2.5 3,033,075 + +0.9 3,033,151 - -0.9 3,033,151 - +1.1 3,033,332 + BPHYT_RS33225 0.23 -2.3 3,033,575 - BPHYT_RS33225 0.49 -3.8 3,033,606 + BPHYT_RS33225 0.52 -2.4 3,033,614 + BPHYT_RS33225 0.53 -2.2 3,033,619 - BPHYT_RS33225 0.53 -1.7 3,033,656 + BPHYT_RS33225 0.57 +0.5 3,033,657 - BPHYT_RS33225 0.57 -2.9 3,033,773 + BPHYT_RS33225 0.70 -2.9 3,033,796 + BPHYT_RS33225 0.72 +0.1 3,034,065 + -2.8 3,034,111 - -0.4 3,034,127 - +1.5 3,034,194 - +2.4 3,034,225 - +2.1 3,034,225 - +1.6 3,034,258 - +1.7 3,034,397 - +1.6 3,034,409 + +0.5 3,034,457 - +1.6 3,034,463 + +0.7 3,034,695 - +1.8 3,034,756 - -0.4 3,034,828 - +0.4 3,034,877 + -0.0 3,034,878 - +0.1 3,034,997 + +1.8 3,034,997 + +1.9 3,034,998 - +0.9 3,034,998 - +1.1 3,034,998 - +1.1
Or see this region's nucleotide sequence