Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33075

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33070 and BPHYT_RS33075 are separated by 128 nucleotidesBPHYT_RS33075 and BPHYT_RS33080 are separated by 816 nucleotides BPHYT_RS33070: BPHYT_RS33070 - haloacid dehalogenase, at 2,997,069 to 2,998,166 _RS33070 BPHYT_RS33075: BPHYT_RS33075 - sulfatase maturase, at 2,998,295 to 2,999,542 _RS33075 BPHYT_RS33080: BPHYT_RS33080 - hypothetical protein, at 3,000,359 to 3,000,769 _RS33080 Position (kb) 2998 2999 3000Strain fitness (log2 ratio) -1 0 1 2at 2997.536 kb on + strand, within BPHYT_RS33070at 2997.573 kb on + strand, within BPHYT_RS33070at 2997.679 kb on + strand, within BPHYT_RS33070at 2997.829 kb on + strand, within BPHYT_RS33070at 2998.149 kb on - strandat 2998.406 kb on + strandat 2998.407 kb on - strandat 2998.500 kb on + strand, within BPHYT_RS33075at 2998.500 kb on + strand, within BPHYT_RS33075at 2998.500 kb on + strand, within BPHYT_RS33075at 2998.914 kb on - strand, within BPHYT_RS33075at 2999.105 kb on + strand, within BPHYT_RS33075at 2999.128 kb on - strand, within BPHYT_RS33075at 2999.711 kb on - strandat 3000.132 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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2,997,536 + BPHYT_RS33070 0.43 +2.7
2,997,573 + BPHYT_RS33070 0.46 -0.9
2,997,679 + BPHYT_RS33070 0.56 +0.2
2,997,829 + BPHYT_RS33070 0.69 +1.5
2,998,149 - +0.3
2,998,406 + +0.1
2,998,407 - -0.4
2,998,500 + BPHYT_RS33075 0.16 +2.1
2,998,500 + BPHYT_RS33075 0.16 -1.6
2,998,500 + BPHYT_RS33075 0.16 -0.7
2,998,914 - BPHYT_RS33075 0.50 -1.6
2,999,105 + BPHYT_RS33075 0.65 -1.1
2,999,128 - BPHYT_RS33075 0.67 -0.5
2,999,711 - +0.1
3,000,132 - +0.2

Or see this region's nucleotide sequence