Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32950 and BPHYT_RS32955 are separated by 468 nucleotides BPHYT_RS32955 and BPHYT_RS32960 are separated by 13 nucleotides BPHYT_RS32960 and BPHYT_RS32965 are separated by 92 nucleotides
BPHYT_RS32950: BPHYT_RS32950 - transposase, at 2,971,779 to 2,972,948
_RS32950
BPHYT_RS32955: BPHYT_RS32955 - polyketide synthase, at 2,973,417 to 2,974,073
_RS32955
BPHYT_RS32960: BPHYT_RS32960 - short-chain dehydrogenase, at 2,974,087 to 2,974,812
_RS32960
BPHYT_RS32965: BPHYT_RS32965 - HxlR family transcriptional regulator, at 2,974,905 to 2,975,300
_RS32965
Position (kb)
2973
2974
2975 Strain fitness (log2 ratio)
-2
-1
0
1 at 2973.291 kb on + strand at 2973.355 kb on + strand at 2973.383 kb on + strand at 2973.383 kb on + strand at 2973.383 kb on + strand at 2973.384 kb on - strand at 2973.418 kb on + strand at 2973.419 kb on - strand at 2973.419 kb on - strand at 2973.429 kb on + strand at 2973.491 kb on + strand, within BPHYT_RS32955 at 2973.703 kb on + strand, within BPHYT_RS32955 at 2973.741 kb on - strand, within BPHYT_RS32955 at 2973.822 kb on + strand, within BPHYT_RS32955 at 2973.914 kb on - strand, within BPHYT_RS32955 at 2973.914 kb on - strand, within BPHYT_RS32955 at 2974.029 kb on - strand at 2974.053 kb on - strand at 2974.073 kb on + strand at 2974.074 kb on - strand at 2974.320 kb on - strand, within BPHYT_RS32960 at 2974.320 kb on - strand, within BPHYT_RS32960 at 2974.833 kb on + strand at 2974.834 kb on - strand at 2974.852 kb on - strand at 2974.852 kb on - strand at 2974.861 kb on - strand at 2974.886 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 2,973,291 + -0.4 2,973,355 + +0.3 2,973,383 + -0.9 2,973,383 + +0.5 2,973,383 + -0.5 2,973,384 - -0.3 2,973,418 + +0.6 2,973,419 - +0.2 2,973,419 - -0.8 2,973,429 + -0.4 2,973,491 + BPHYT_RS32955 0.11 +0.5 2,973,703 + BPHYT_RS32955 0.44 +0.3 2,973,741 - BPHYT_RS32955 0.49 -1.3 2,973,822 + BPHYT_RS32955 0.62 -1.6 2,973,914 - BPHYT_RS32955 0.76 -0.5 2,973,914 - BPHYT_RS32955 0.76 +0.0 2,974,029 - +0.1 2,974,053 - -0.2 2,974,073 + -2.4 2,974,074 - -0.4 2,974,320 - BPHYT_RS32960 0.32 +1.4 2,974,320 - BPHYT_RS32960 0.32 +0.6 2,974,833 + +1.1 2,974,834 - +0.3 2,974,852 - -0.2 2,974,852 - +0.3 2,974,861 - -1.7 2,974,886 - +0.3
Or see this region's nucleotide sequence