Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32370

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32365 and BPHYT_RS32370 are separated by 323 nucleotides BPHYT_RS32365: BPHYT_RS32365 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,832,193 to 2,833,260 _RS32365 BPHYT_RS32370: BPHYT_RS32370 - LysR family transcriptional regulator, at 2,833,584 to 2,834,501 _RS32370 Position (kb) 2833 2834 2835Strain fitness (log2 ratio) -2 -1 0 1 2at 2832.880 kb on + strand, within BPHYT_RS32365at 2832.880 kb on + strand, within BPHYT_RS32365at 2832.897 kb on - strand, within BPHYT_RS32365at 2833.227 kb on + strandat 2833.380 kb on + strandat 2833.487 kb on + strandat 2833.487 kb on + strandat 2833.487 kb on + strandat 2833.487 kb on + strandat 2833.487 kb on + strandat 2833.488 kb on - strandat 2833.489 kb on + strandat 2833.489 kb on + strandat 2833.490 kb on - strandat 2833.496 kb on + strandat 2833.788 kb on + strand, within BPHYT_RS32370at 2833.921 kb on + strandat 2834.032 kb on + strand, within BPHYT_RS32370at 2834.036 kb on + strand, within BPHYT_RS32370at 2834.036 kb on + strand, within BPHYT_RS32370at 2834.056 kb on - strand, within BPHYT_RS32370at 2834.056 kb on - strand, within BPHYT_RS32370at 2834.056 kb on - strand, within BPHYT_RS32370at 2834.057 kb on + strand, within BPHYT_RS32370at 2834.275 kb on + strand, within BPHYT_RS32370at 2834.276 kb on - strand, within BPHYT_RS32370at 2834.323 kb on + strand, within BPHYT_RS32370at 2834.636 kb on + strandat 2834.637 kb on - strandat 2834.967 kb on + strandat 2834.967 kb on + strandat 2835.025 kb on - strandat 2835.179 kb on - strandat 2835.179 kb on - strandat 2835.269 kb on + strandat 2835.318 kb on + strandat 2835.421 kb on - strandat 2835.421 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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2,832,880 + BPHYT_RS32365 0.64 +0.1
2,832,880 + BPHYT_RS32365 0.64 -0.8
2,832,897 - BPHYT_RS32365 0.66 -1.2
2,833,227 + -0.7
2,833,380 + -0.4
2,833,487 + +0.9
2,833,487 + -0.1
2,833,487 + +0.2
2,833,487 + +1.9
2,833,487 + -0.5
2,833,488 - -1.0
2,833,489 + -0.2
2,833,489 + -0.5
2,833,490 - +0.7
2,833,496 + +0.8
2,833,788 + BPHYT_RS32370 0.22 +1.3
2,833,921 + -0.2
2,834,032 + BPHYT_RS32370 0.49 -1.2
2,834,036 + BPHYT_RS32370 0.49 -0.6
2,834,036 + BPHYT_RS32370 0.49 -0.1
2,834,056 - BPHYT_RS32370 0.51 -1.5
2,834,056 - BPHYT_RS32370 0.51 -1.0
2,834,056 - BPHYT_RS32370 0.51 +0.5
2,834,057 + BPHYT_RS32370 0.52 +0.0
2,834,275 + BPHYT_RS32370 0.75 -0.7
2,834,276 - BPHYT_RS32370 0.75 -1.6
2,834,323 + BPHYT_RS32370 0.81 -1.1
2,834,636 + -0.8
2,834,637 - -0.1
2,834,967 + +0.8
2,834,967 + +1.0
2,835,025 - +0.1
2,835,179 - -0.1
2,835,179 - -2.1
2,835,269 + +0.3
2,835,318 + +0.5
2,835,421 - +0.6
2,835,421 - -2.0

Or see this region's nucleotide sequence