Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28275 and BPHYT_RS28280 are separated by 191 nucleotides BPHYT_RS28280 and BPHYT_RS28285 are separated by 123 nucleotides BPHYT_RS28285 and BPHYT_RS28290 are separated by 382 nucleotides BPHYT_RS28290 and BPHYT_RS28295 are separated by 59 nucleotides
BPHYT_RS28275: BPHYT_RS28275 - glycerol acyltransferase, at 1,900,169 to 1,900,879
_RS28275
BPHYT_RS28280: BPHYT_RS28280 - hypothetical protein, at 1,901,071 to 1,901,385
_RS28280
BPHYT_RS28285: BPHYT_RS28285 - MFS transporter, at 1,901,509 to 1,902,756
_RS28285
BPHYT_RS28290: BPHYT_RS28290 - hypothetical protein, at 1,903,139 to 1,903,411
_RS28290
BPHYT_RS28295: BPHYT_RS28295 - hypothetical protein, at 1,903,471 to 1,903,788
_RS28295
Position (kb) 1901
1902
1903 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1900.850 kb on - strand at 1900.950 kb on + strand at 1900.951 kb on - strand at 1900.951 kb on - strand at 1901.305 kb on + strand, within BPHYT_RS28280 at 1901.305 kb on + strand, within BPHYT_RS28280 at 1902.026 kb on + strand, within BPHYT_RS28285 at 1902.756 kb on + strand at 1902.756 kb on + strand at 1902.757 kb on - strand at 1902.757 kb on - strand at 1902.759 kb on + strand at 1902.774 kb on + strand at 1902.774 kb on + strand at 1902.778 kb on - strand at 1902.778 kb on - strand at 1902.932 kb on + strand at 1902.932 kb on + strand at 1902.991 kb on - strand at 1903.145 kb on - strand at 1903.145 kb on - strand at 1903.205 kb on + strand, within BPHYT_RS28290 at 1903.206 kb on - strand, within BPHYT_RS28290 at 1903.484 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 1,900,850 - +0.6 1,900,950 + +0.2 1,900,951 - +1.1 1,900,951 - +1.3 1,901,305 + BPHYT_RS28280 0.74 +1.9 1,901,305 + BPHYT_RS28280 0.74 +0.5 1,902,026 + BPHYT_RS28285 0.41 -2.9 1,902,756 + +0.8 1,902,756 + +0.2 1,902,757 - -0.8 1,902,757 - -0.0 1,902,759 + +0.9 1,902,774 + -1.0 1,902,774 + -0.3 1,902,778 - -1.7 1,902,778 - -0.9 1,902,932 + +0.3 1,902,932 + +2.3 1,902,991 - -0.7 1,903,145 - +0.4 1,903,145 - -0.5 1,903,205 + BPHYT_RS28290 0.24 -2.4 1,903,206 - BPHYT_RS28290 0.25 +1.3 1,903,484 + +0.7
Or see this region's nucleotide sequence