Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25830 and BPHYT_RS25835 are separated by 433 nucleotides BPHYT_RS25835 and BPHYT_RS25840 are separated by 270 nucleotides BPHYT_RS25840 and BPHYT_RS25845 are separated by 18 nucleotides
BPHYT_RS25830: BPHYT_RS25830 - sugar ABC transporter substrate-binding protein, at 1,369,527 to 1,370,672
_RS25830
BPHYT_RS25835: BPHYT_RS25835 - hypothetical protein, at 1,371,106 to 1,371,393
_RS25835
BPHYT_RS25840: BPHYT_RS25840 - acriflavin resistance protein, at 1,371,664 to 1,374,918
_RS25840
BPHYT_RS25845: BPHYT_RS25845 - RND transporter MFP subunit, at 1,374,937 to 1,376,304
_RS25845
Position (kb) 1371
1372
1373
1374
1375 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1370.774 kb on + strand at 1370.774 kb on + strand at 1370.778 kb on - strand at 1370.814 kb on + strand at 1370.815 kb on - strand at 1370.818 kb on + strand at 1371.544 kb on + strand at 1371.545 kb on - strand at 1372.053 kb on + strand, within BPHYT_RS25840 at 1372.053 kb on + strand, within BPHYT_RS25840 at 1372.053 kb on + strand, within BPHYT_RS25840 at 1372.058 kb on - strand, within BPHYT_RS25840 at 1372.058 kb on - strand, within BPHYT_RS25840 at 1372.058 kb on - strand, within BPHYT_RS25840 at 1372.227 kb on + strand, within BPHYT_RS25840 at 1372.342 kb on + strand, within BPHYT_RS25840 at 1372.342 kb on + strand, within BPHYT_RS25840 at 1373.531 kb on - strand, within BPHYT_RS25840 at 1373.611 kb on + strand, within BPHYT_RS25840 at 1374.228 kb on + strand, within BPHYT_RS25840 at 1374.335 kb on + strand, within BPHYT_RS25840 at 1375.382 kb on - strand, within BPHYT_RS25845
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 1,370,774 + +0.4 1,370,774 + -0.6 1,370,778 - +2.5 1,370,814 + -0.9 1,370,815 - -1.1 1,370,818 + +0.2 1,371,544 + +2.2 1,371,545 - -0.4 1,372,053 + BPHYT_RS25840 0.12 +0.3 1,372,053 + BPHYT_RS25840 0.12 -0.8 1,372,053 + BPHYT_RS25840 0.12 +1.0 1,372,058 - BPHYT_RS25840 0.12 -1.9 1,372,058 - BPHYT_RS25840 0.12 -2.5 1,372,058 - BPHYT_RS25840 0.12 -0.1 1,372,227 + BPHYT_RS25840 0.17 +0.3 1,372,342 + BPHYT_RS25840 0.21 -3.3 1,372,342 + BPHYT_RS25840 0.21 -0.3 1,373,531 - BPHYT_RS25840 0.57 -2.0 1,373,611 + BPHYT_RS25840 0.60 -0.2 1,374,228 + BPHYT_RS25840 0.79 -0.9 1,374,335 + BPHYT_RS25840 0.82 +0.2 1,375,382 - BPHYT_RS25845 0.33 -1.0
Or see this region's nucleotide sequence