Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25225

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25220 and BPHYT_RS25225 are separated by 136 nucleotidesBPHYT_RS25225 and BPHYT_RS25230 are separated by 96 nucleotides BPHYT_RS25220: BPHYT_RS25220 - ferredoxin oxidoreductase, at 1,232,139 to 1,233,386 _RS25220 BPHYT_RS25225: BPHYT_RS25225 - glycine/betaine ABC transporter substrate-binding protein, at 1,233,523 to 1,234,380 _RS25225 BPHYT_RS25230: BPHYT_RS25230 - formyltetrahydrofolate deformylase, at 1,234,477 to 1,235,352 _RS25230 Position (kb) 1233 1234 1235Strain fitness (log2 ratio) -2 -1 0 1at 1232.666 kb on + strand, within BPHYT_RS25220at 1232.895 kb on - strand, within BPHYT_RS25220at 1233.234 kb on - strand, within BPHYT_RS25220at 1233.380 kb on + strandat 1234.136 kb on - strand, within BPHYT_RS25225at 1234.479 kb on - strandat 1234.601 kb on + strand, within BPHYT_RS25230at 1234.817 kb on + strand, within BPHYT_RS25230at 1234.817 kb on + strand, within BPHYT_RS25230at 1235.247 kb on - strand, within BPHYT_RS25230

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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1,232,666 + BPHYT_RS25220 0.42 -2.0
1,232,895 - BPHYT_RS25220 0.61 +0.1
1,233,234 - BPHYT_RS25220 0.88 -1.2
1,233,380 + -0.6
1,234,136 - BPHYT_RS25225 0.71 +0.6
1,234,479 - -0.3
1,234,601 + BPHYT_RS25230 0.14 +0.0
1,234,817 + BPHYT_RS25230 0.39 -1.3
1,234,817 + BPHYT_RS25230 0.39 -1.4
1,235,247 - BPHYT_RS25230 0.88 -1.0

Or see this region's nucleotide sequence