Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24530 and BPHYT_RS24535 are separated by 27 nucleotides BPHYT_RS24535 and BPHYT_RS24540 are separated by 19 nucleotides BPHYT_RS24540 and BPHYT_RS24545 are separated by 152 nucleotides
BPHYT_RS24530: BPHYT_RS24530 - formate dehydrogenase, at 1,070,688 to 1,073,612
_RS24530
BPHYT_RS24535: BPHYT_RS24535 - ferredoxin, at 1,073,640 to 1,074,395
_RS24535
BPHYT_RS24540: BPHYT_RS24540 - DMSO reductase, at 1,074,415 to 1,075,365
_RS24540
BPHYT_RS24545: BPHYT_RS24545 - ribonucleoside-diphosphate reductase, at 1,075,518 to 1,078,103
_RS24545
Position (kb)
1074
1075
1076 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1073.611 kb on - strand at 1074.545 kb on - strand, within BPHYT_RS24540 at 1074.545 kb on - strand, within BPHYT_RS24540 at 1074.573 kb on - strand, within BPHYT_RS24540 at 1074.715 kb on + strand, within BPHYT_RS24540 at 1075.424 kb on - strand at 1075.455 kb on - strand at 1075.622 kb on + strand at 1075.622 kb on + strand at 1075.622 kb on + strand at 1076.122 kb on + strand, within BPHYT_RS24545 at 1076.302 kb on + strand, within BPHYT_RS24545 at 1076.303 kb on - strand, within BPHYT_RS24545
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 1,073,611 - +0.5 1,074,545 - BPHYT_RS24540 0.14 +1.1 1,074,545 - BPHYT_RS24540 0.14 -2.1 1,074,573 - BPHYT_RS24540 0.17 -1.4 1,074,715 + BPHYT_RS24540 0.32 -1.2 1,075,424 - +2.3 1,075,455 - -0.5 1,075,622 + +4.1 1,075,622 + +0.4 1,075,622 + +0.1 1,076,122 + BPHYT_RS24545 0.23 -0.7 1,076,302 + BPHYT_RS24545 0.30 +1.6 1,076,303 - BPHYT_RS24545 0.30 -2.2
Or see this region's nucleotide sequence