Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24345

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24335 and BPHYT_RS24340 are separated by 2 nucleotidesBPHYT_RS24340 and BPHYT_RS24345 are separated by 282 nucleotidesBPHYT_RS24345 and BPHYT_RS24350 are separated by 45 nucleotidesBPHYT_RS24350 and BPHYT_RS24355 are separated by 99 nucleotides BPHYT_RS24335: BPHYT_RS24335 - toxin HipA, at 1,025,758 to 1,027,098 _RS24335 BPHYT_RS24340: BPHYT_RS24340 - XRE family transcriptional regulator, at 1,027,101 to 1,027,355 _RS24340 BPHYT_RS24345: BPHYT_RS24345 - FAD-dependent oxidoreductase, at 1,027,638 to 1,028,978 _RS24345 BPHYT_RS24350: BPHYT_RS24350 - hypothetical protein, at 1,029,024 to 1,029,656 _RS24350 BPHYT_RS24355: BPHYT_RS24355 - hypothetical protein, at 1,029,756 to 1,030,007 _RS24355 Position (kb) 1027 1028 1029Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1026.840 kb on - strand, within BPHYT_RS24335at 1026.999 kb on - strandat 1027.483 kb on - strandat 1027.502 kb on - strandat 1027.622 kb on - strandat 1027.622 kb on - strandat 1027.749 kb on + strandat 1027.752 kb on + strandat 1027.752 kb on + strandat 1027.753 kb on - strandat 1027.753 kb on - strandat 1027.753 kb on - strandat 1027.753 kb on - strandat 1029.060 kb on + strandat 1029.060 kb on + strandat 1029.330 kb on + strand, within BPHYT_RS24350at 1029.585 kb on + strand, within BPHYT_RS24350at 1029.586 kb on - strand, within BPHYT_RS24350at 1029.768 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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1,026,840 - BPHYT_RS24335 0.81 +0.2
1,026,999 - +0.7
1,027,483 - -2.8
1,027,502 - +0.1
1,027,622 - +0.4
1,027,622 - -1.0
1,027,749 + +0.5
1,027,752 + +2.1
1,027,752 + +0.7
1,027,753 - -0.1
1,027,753 - +0.7
1,027,753 - -2.5
1,027,753 - +0.1
1,029,060 + -1.5
1,029,060 + -0.5
1,029,330 + BPHYT_RS24350 0.48 -0.4
1,029,585 + BPHYT_RS24350 0.89 -0.7
1,029,586 - BPHYT_RS24350 0.89 -1.2
1,029,768 + -0.6

Or see this region's nucleotide sequence