Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22960 and BPHYT_RS22965 are separated by 74 nucleotides BPHYT_RS22965 and BPHYT_RS22970 are separated by 82 nucleotides BPHYT_RS22970 and BPHYT_RS22975 are separated by 273 nucleotides
BPHYT_RS22960: BPHYT_RS22960 - peptidase, at 708,607 to 710,145
_RS22960
BPHYT_RS22965: BPHYT_RS22965 - ligand-gated channel protein, at 710,220 to 712,634
_RS22965
BPHYT_RS22970: BPHYT_RS22970 - hypothetical protein, at 712,717 to 713,127
_RS22970
BPHYT_RS22975: BPHYT_RS22975 - hypothetical protein, at 713,401 to 715,545
_RS22975
Position (kb) 710
711
712
713 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 709.994 kb on + strand at 709.995 kb on - strand at 710.273 kb on - strand at 710.341 kb on + strand at 710.483 kb on - strand, within BPHYT_RS22965 at 710.527 kb on + strand, within BPHYT_RS22965 at 710.767 kb on - strand, within BPHYT_RS22965 at 710.808 kb on + strand, within BPHYT_RS22965 at 710.808 kb on + strand, within BPHYT_RS22965 at 710.997 kb on - strand, within BPHYT_RS22965 at 710.998 kb on + strand, within BPHYT_RS22965 at 711.224 kb on - strand, within BPHYT_RS22965 at 711.284 kb on - strand, within BPHYT_RS22965 at 711.521 kb on - strand, within BPHYT_RS22965 at 711.553 kb on + strand at 711.758 kb on - strand, within BPHYT_RS22965 at 711.758 kb on - strand, within BPHYT_RS22965 at 711.763 kb on + strand, within BPHYT_RS22965 at 712.052 kb on - strand, within BPHYT_RS22965 at 712.072 kb on + strand, within BPHYT_RS22965 at 712.135 kb on + strand, within BPHYT_RS22965 at 712.177 kb on + strand, within BPHYT_RS22965 at 712.279 kb on + strand, within BPHYT_RS22965 at 712.279 kb on + strand, within BPHYT_RS22965 at 712.279 kb on + strand, within BPHYT_RS22965 at 712.280 kb on - strand, within BPHYT_RS22965 at 712.280 kb on - strand, within BPHYT_RS22965 at 712.538 kb on - strand at 713.139 kb on + strand at 713.140 kb on - strand at 713.157 kb on + strand at 713.195 kb on + strand at 713.272 kb on + strand at 713.272 kb on + strand at 713.273 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 709,994 + -1.7 709,995 - -0.0 710,273 - -1.9 710,341 + +1.1 710,483 - BPHYT_RS22965 0.11 +0.6 710,527 + BPHYT_RS22965 0.13 -0.6 710,767 - BPHYT_RS22965 0.23 -0.5 710,808 + BPHYT_RS22965 0.24 +1.0 710,808 + BPHYT_RS22965 0.24 -0.2 710,997 - BPHYT_RS22965 0.32 +0.2 710,998 + BPHYT_RS22965 0.32 -0.5 711,224 - BPHYT_RS22965 0.42 -0.7 711,284 - BPHYT_RS22965 0.44 +0.0 711,521 - BPHYT_RS22965 0.54 -0.1 711,553 + +4.5 711,758 - BPHYT_RS22965 0.64 -0.7 711,758 - BPHYT_RS22965 0.64 +0.4 711,763 + BPHYT_RS22965 0.64 +0.9 712,052 - BPHYT_RS22965 0.76 +0.5 712,072 + BPHYT_RS22965 0.77 -0.2 712,135 + BPHYT_RS22965 0.79 -3.4 712,177 + BPHYT_RS22965 0.81 -2.5 712,279 + BPHYT_RS22965 0.85 -1.0 712,279 + BPHYT_RS22965 0.85 -0.3 712,279 + BPHYT_RS22965 0.85 +1.2 712,280 - BPHYT_RS22965 0.85 +0.3 712,280 - BPHYT_RS22965 0.85 -0.2 712,538 - -0.4 713,139 + +0.4 713,140 - -0.2 713,157 + +0.3 713,195 + -0.4 713,272 + -0.2 713,272 + -1.0 713,273 - -0.7
Or see this region's nucleotide sequence