Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19500 and BPHYT_RS19505 are separated by 145 nucleotides BPHYT_RS19505 and BPHYT_RS19510 are separated by 315 nucleotides BPHYT_RS19510 and BPHYT_RS19515 are separated by 53 nucleotides
BPHYT_RS19500: BPHYT_RS19500 - methylmalonate-semialdehyde dehydrogenase, at 4,388,532 to 4,390,070
_RS19500
BPHYT_RS19505: BPHYT_RS19505 - LysR family transcriptional regulator, at 4,390,216 to 4,391,115
_RS19505
BPHYT_RS19510: BPHYT_RS19510 - adenylate cyclase, at 4,391,431 to 4,391,967
_RS19510
BPHYT_RS19515: BPHYT_RS19515 - AsnC family transcriptional regulator, at 4,392,021 to 4,392,512
_RS19515
Position (kb) 4390
4391
4392 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4389.326 kb on - strand, within BPHYT_RS19500 at 4389.331 kb on + strand, within BPHYT_RS19500 at 4389.332 kb on - strand, within BPHYT_RS19500 at 4389.332 kb on - strand, within BPHYT_RS19500 at 4389.721 kb on - strand at 4389.721 kb on - strand, within BPHYT_RS19500 at 4390.057 kb on + strand at 4390.057 kb on + strand at 4390.057 kb on + strand at 4390.058 kb on - strand at 4390.176 kb on + strand at 4390.177 kb on - strand at 4390.177 kb on - strand at 4390.192 kb on + strand at 4390.192 kb on + strand at 4390.194 kb on + strand at 4390.194 kb on + strand at 4390.195 kb on - strand at 4390.481 kb on + strand, within BPHYT_RS19505 at 4390.481 kb on + strand, within BPHYT_RS19505 at 4390.482 kb on - strand, within BPHYT_RS19505 at 4390.648 kb on + strand, within BPHYT_RS19505 at 4390.651 kb on + strand, within BPHYT_RS19505 at 4390.651 kb on + strand, within BPHYT_RS19505 at 4390.651 kb on + strand, within BPHYT_RS19505 at 4390.652 kb on - strand, within BPHYT_RS19505 at 4390.652 kb on - strand, within BPHYT_RS19505 at 4390.652 kb on - strand, within BPHYT_RS19505 at 4390.833 kb on + strand, within BPHYT_RS19505 at 4391.037 kb on + strand at 4391.038 kb on - strand at 4391.171 kb on + strand at 4391.172 kb on - strand at 4391.653 kb on + strand, within BPHYT_RS19510 at 4391.654 kb on - strand, within BPHYT_RS19510 at 4391.654 kb on - strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.750 kb on - strand, within BPHYT_RS19510 at 4391.750 kb on - strand, within BPHYT_RS19510 at 4391.750 kb on - strand, within BPHYT_RS19510 at 4391.795 kb on + strand, within BPHYT_RS19510 at 4391.795 kb on + strand, within BPHYT_RS19510 at 4391.965 kb on + strand at 4391.965 kb on + strand at 4392.105 kb on + strand, within BPHYT_RS19515 at 4392.106 kb on - strand, within BPHYT_RS19515
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 4,389,326 - BPHYT_RS19500 0.52 +1.1 4,389,331 + BPHYT_RS19500 0.52 +0.8 4,389,332 - BPHYT_RS19500 0.52 -0.0 4,389,332 - BPHYT_RS19500 0.52 +0.9 4,389,721 - +1.2 4,389,721 - BPHYT_RS19500 0.77 -0.6 4,390,057 + -0.2 4,390,057 + -1.7 4,390,057 + +0.5 4,390,058 - -0.4 4,390,176 + -0.9 4,390,177 - +1.3 4,390,177 - -0.3 4,390,192 + -0.4 4,390,192 + +1.1 4,390,194 + -0.3 4,390,194 + -0.3 4,390,195 - -1.3 4,390,481 + BPHYT_RS19505 0.29 +1.6 4,390,481 + BPHYT_RS19505 0.29 +0.5 4,390,482 - BPHYT_RS19505 0.30 +0.3 4,390,648 + BPHYT_RS19505 0.48 +0.3 4,390,651 + BPHYT_RS19505 0.48 +0.3 4,390,651 + BPHYT_RS19505 0.48 +2.1 4,390,651 + BPHYT_RS19505 0.48 +0.5 4,390,652 - BPHYT_RS19505 0.48 +0.3 4,390,652 - BPHYT_RS19505 0.48 +0.1 4,390,652 - BPHYT_RS19505 0.48 +0.5 4,390,833 + BPHYT_RS19505 0.69 +1.4 4,391,037 + -0.2 4,391,038 - -0.3 4,391,171 + -0.3 4,391,172 - +0.8 4,391,653 + BPHYT_RS19510 0.41 -0.9 4,391,654 - BPHYT_RS19510 0.42 +0.1 4,391,654 - BPHYT_RS19510 0.42 -0.5 4,391,749 + BPHYT_RS19510 0.59 -1.1 4,391,749 + BPHYT_RS19510 0.59 +0.3 4,391,749 + BPHYT_RS19510 0.59 -1.8 4,391,749 + BPHYT_RS19510 0.59 -0.5 4,391,749 + BPHYT_RS19510 0.59 -0.4 4,391,750 - BPHYT_RS19510 0.59 +1.5 4,391,750 - BPHYT_RS19510 0.59 -0.2 4,391,750 - BPHYT_RS19510 0.59 +0.8 4,391,795 + BPHYT_RS19510 0.68 +0.3 4,391,795 + BPHYT_RS19510 0.68 +0.1 4,391,965 + +0.4 4,391,965 + -0.1 4,392,105 + BPHYT_RS19515 0.17 -0.4 4,392,106 - BPHYT_RS19515 0.17 -0.3
Or see this region's nucleotide sequence