Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14405 and BPHYT_RS14410 are separated by 101 nucleotides BPHYT_RS14410 and BPHYT_RS14415 are separated by 117 nucleotides BPHYT_RS14415 and BPHYT_RS14420 are separated by 232 nucleotides BPHYT_RS14420 and BPHYT_RS14425 are separated by 152 nucleotides
BPHYT_RS14405: BPHYT_RS14405 - RNA polymerase sigma factor RpoE, at 3,287,548 to 3,288,147
_RS14405
BPHYT_RS14410: BPHYT_RS14410 - hypothetical protein, at 3,288,249 to 3,288,632
_RS14410
BPHYT_RS14415: BPHYT_RS14415 - 3-oxoacyl-ACP synthase, at 3,288,750 to 3,289,988
_RS14415
BPHYT_RS14420: BPHYT_RS14420 - acyl carrier protein, at 3,290,221 to 3,290,460
_RS14420
BPHYT_RS14425: BPHYT_RS14425 - 3-ketoacyl-ACP reductase, at 3,290,613 to 3,291,362
_RS14425
Position (kb)
3288
3289
3290 Strain fitness (log2 ratio)
-1
0
1
2 at 3288.193 kb on - strand at 3288.193 kb on - strand at 3288.218 kb on - strand at 3288.218 kb on - strand at 3288.324 kb on + strand, within BPHYT_RS14410 at 3288.494 kb on - strand, within BPHYT_RS14410 at 3288.546 kb on - strand, within BPHYT_RS14410 at 3288.583 kb on + strand, within BPHYT_RS14410 at 3290.568 kb on - strand at 3290.576 kb on - strand at 3290.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 3,288,193 - +0.4 3,288,193 - +2.6 3,288,218 - +0.6 3,288,218 - +0.8 3,288,324 + BPHYT_RS14410 0.20 +2.2 3,288,494 - BPHYT_RS14410 0.64 -0.6 3,288,546 - BPHYT_RS14410 0.77 -0.0 3,288,583 + BPHYT_RS14410 0.87 -1.1 3,290,568 - +0.8 3,290,576 - -0.3 3,290,598 - -1.4
Or see this region's nucleotide sequence