Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14395 and BPHYT_RS14400 are separated by 4 nucleotides BPHYT_RS14400 and BPHYT_RS14405 are separated by 83 nucleotides BPHYT_RS14405 and BPHYT_RS14410 are separated by 101 nucleotides BPHYT_RS14410 and BPHYT_RS14415 are separated by 117 nucleotides
BPHYT_RS14395: BPHYT_RS14395 - sugar dehydratase, at 3,285,787 to 3,286,830
_RS14395
BPHYT_RS14400: BPHYT_RS14400 - anti-sigma factor, at 3,286,835 to 3,287,464
_RS14400
BPHYT_RS14405: BPHYT_RS14405 - RNA polymerase sigma factor RpoE, at 3,287,548 to 3,288,147
_RS14405
BPHYT_RS14410: BPHYT_RS14410 - hypothetical protein, at 3,288,249 to 3,288,632
_RS14410
BPHYT_RS14415: BPHYT_RS14415 - 3-oxoacyl-ACP synthase, at 3,288,750 to 3,289,988
_RS14415
Position (kb)
3287
3288
3289 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3286.746 kb on + strand at 3286.801 kb on - strand at 3286.836 kb on + strand at 3286.836 kb on + strand at 3286.930 kb on - strand, within BPHYT_RS14400 at 3287.314 kb on - strand, within BPHYT_RS14400 at 3287.314 kb on - strand, within BPHYT_RS14400 at 3287.357 kb on - strand, within BPHYT_RS14400 at 3287.479 kb on + strand at 3287.479 kb on + strand at 3287.480 kb on - strand at 3287.480 kb on - strand at 3287.485 kb on + strand at 3287.485 kb on + strand at 3287.486 kb on - strand at 3287.514 kb on - strand at 3288.193 kb on - strand at 3288.193 kb on - strand at 3288.218 kb on - strand at 3288.218 kb on - strand at 3288.324 kb on + strand, within BPHYT_RS14410 at 3288.494 kb on - strand, within BPHYT_RS14410 at 3288.546 kb on - strand, within BPHYT_RS14410 at 3288.583 kb on + strand, within BPHYT_RS14410
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 3,286,746 + +1.6 3,286,801 - +2.3 3,286,836 + +1.9 3,286,836 + +1.8 3,286,930 - BPHYT_RS14400 0.15 +1.2 3,287,314 - BPHYT_RS14400 0.76 +1.6 3,287,314 - BPHYT_RS14400 0.76 +2.5 3,287,357 - BPHYT_RS14400 0.83 +3.8 3,287,479 + +1.6 3,287,479 + +1.9 3,287,480 - +0.7 3,287,480 - +0.8 3,287,485 + +2.3 3,287,485 + +2.3 3,287,486 - +0.3 3,287,514 - -1.3 3,288,193 - +0.4 3,288,193 - +2.6 3,288,218 - +0.6 3,288,218 - +0.8 3,288,324 + BPHYT_RS14410 0.20 +2.2 3,288,494 - BPHYT_RS14410 0.64 -0.6 3,288,546 - BPHYT_RS14410 0.77 -0.0 3,288,583 + BPHYT_RS14410 0.87 -1.1
Or see this region's nucleotide sequence