Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14340 and BPHYT_RS14345 are separated by 100 nucleotides BPHYT_RS14345 and BPHYT_RS14350 are separated by 69 nucleotides BPHYT_RS14350 and BPHYT_RS14355 are separated by 15 nucleotides
BPHYT_RS14340: BPHYT_RS14340 - Fis family transcriptional regulator, at 3,273,972 to 3,275,354
_RS14340
BPHYT_RS14345: BPHYT_RS14345 - beta-hexosaminidase, at 3,275,455 to 3,276,483
_RS14345
BPHYT_RS14350: BPHYT_RS14350 - holo-[acyl-carrier-protein] synthase, at 3,276,553 to 3,276,975
_RS14350
BPHYT_RS14355: BPHYT_RS14355 - pyridoxine 5'-phosphate synthase, at 3,276,991 to 3,277,755
_RS14355
Position (kb)
3275
3276
3277 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3275.061 kb on + strand, within BPHYT_RS14340 at 3275.062 kb on - strand, within BPHYT_RS14340 at 3275.080 kb on + strand, within BPHYT_RS14340 at 3275.080 kb on + strand, within BPHYT_RS14340 at 3275.080 kb on + strand, within BPHYT_RS14340 at 3275.080 kb on + strand, within BPHYT_RS14340 at 3275.081 kb on - strand, within BPHYT_RS14340 at 3275.112 kb on + strand, within BPHYT_RS14340 at 3275.320 kb on + strand at 3275.320 kb on + strand at 3275.320 kb on + strand at 3275.320 kb on + strand at 3275.321 kb on - strand at 3275.322 kb on + strand at 3275.322 kb on + strand at 3275.322 kb on + strand at 3275.339 kb on + strand at 3275.339 kb on + strand at 3275.339 kb on + strand at 3275.340 kb on - strand at 3275.340 kb on - strand at 3275.340 kb on - strand at 3275.353 kb on - strand at 3275.407 kb on + strand at 3275.407 kb on + strand at 3275.456 kb on + strand at 3275.495 kb on + strand at 3275.873 kb on + strand, within BPHYT_RS14345 at 3276.093 kb on - strand, within BPHYT_RS14345 at 3276.161 kb on + strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.549 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 3,274,779 - BPHYT_RS14340 0.58 +0.6 3,274,779 - BPHYT_RS14340 0.58 +3.2 3,274,779 - BPHYT_RS14340 0.58 +0.1 3,274,779 - BPHYT_RS14340 0.58 +1.2 3,274,779 - BPHYT_RS14340 0.58 +0.9 3,275,061 + BPHYT_RS14340 0.79 +1.1 3,275,062 - BPHYT_RS14340 0.79 +0.6 3,275,080 + BPHYT_RS14340 0.80 +0.9 3,275,080 + BPHYT_RS14340 0.80 +0.7 3,275,080 + BPHYT_RS14340 0.80 +1.3 3,275,080 + BPHYT_RS14340 0.80 +0.8 3,275,081 - BPHYT_RS14340 0.80 +0.6 3,275,112 + BPHYT_RS14340 0.82 +0.4 3,275,320 + +1.5 3,275,320 + +0.0 3,275,320 + +0.9 3,275,320 + +0.9 3,275,321 - +0.5 3,275,322 + +2.5 3,275,322 + +0.6 3,275,322 + +0.6 3,275,339 + +0.6 3,275,339 + -2.6 3,275,339 + +0.7 3,275,340 - +0.0 3,275,340 - +1.3 3,275,340 - +1.0 3,275,353 - +0.1 3,275,407 + -0.7 3,275,407 + -1.1 3,275,456 + -0.1 3,275,495 + -0.9 3,275,873 + BPHYT_RS14345 0.41 +2.2 3,276,093 - BPHYT_RS14345 0.62 +1.6 3,276,161 + BPHYT_RS14345 0.69 +1.0 3,276,162 - BPHYT_RS14345 0.69 -0.1 3,276,162 - BPHYT_RS14345 0.69 -1.5 3,276,162 - BPHYT_RS14345 0.69 +1.2 3,276,549 - +3.0
Or see this region's nucleotide sequence