Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11930

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11925 and BPHYT_RS11930 are separated by 10 nucleotidesBPHYT_RS11930 and BPHYT_RS11935 are separated by 18 nucleotides BPHYT_RS11925: BPHYT_RS11925 - membrane protein, at 2,712,602 to 2,713,873 _RS11925 BPHYT_RS11930: BPHYT_RS11930 - 3-hydroxybutyrate dehydrogenase, at 2,713,884 to 2,714,666 _RS11930 BPHYT_RS11935: BPHYT_RS11935 - acetoacetate decarboxylase, at 2,714,685 to 2,715,464 _RS11935 Position (kb) 2713 2714 2715Strain fitness (log2 ratio) -3 -2 -1 0 1at 2713.380 kb on + strand, within BPHYT_RS11925at 2713.411 kb on + strand, within BPHYT_RS11925at 2713.416 kb on + strand, within BPHYT_RS11925at 2713.417 kb on - strand, within BPHYT_RS11925at 2713.420 kb on - strand, within BPHYT_RS11925at 2713.754 kb on + strandat 2714.050 kb on - strand, within BPHYT_RS11930at 2714.050 kb on - strandat 2714.252 kb on - strand, within BPHYT_RS11930at 2714.728 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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2,713,380 + BPHYT_RS11925 0.61 -1.4
2,713,411 + BPHYT_RS11925 0.64 -0.2
2,713,416 + BPHYT_RS11925 0.64 +0.8
2,713,417 - BPHYT_RS11925 0.64 +0.6
2,713,420 - BPHYT_RS11925 0.64 +0.0
2,713,754 + -0.5
2,714,050 - BPHYT_RS11930 0.21 -0.8
2,714,050 - +0.6
2,714,252 - BPHYT_RS11930 0.47 -3.1
2,714,728 + +1.2

Or see this region's nucleotide sequence