Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11060

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11050 and BPHYT_RS11055 are separated by 39 nucleotidesBPHYT_RS11055 and BPHYT_RS11060 are separated by 133 nucleotidesBPHYT_RS11060 and BPHYT_RS11065 are separated by 241 nucleotides BPHYT_RS11050: BPHYT_RS11050 - molecular chaperone, at 2,509,287 to 2,510,537 _RS11050 BPHYT_RS11055: BPHYT_RS11055 - cobyric acid synthase, at 2,510,577 to 2,511,341 _RS11055 BPHYT_RS11060: BPHYT_RS11060 - 4-hydroxybenzoate 3-monooxygenase, at 2,511,475 to 2,512,653 _RS11060 BPHYT_RS11065: BPHYT_RS11065 - threonine dehydratase, at 2,512,895 to 2,514,445 _RS11065 Position (kb) 2511 2512 2513Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2510.854 kb on + strand, within BPHYT_RS11055at 2510.974 kb on + strand, within BPHYT_RS11055at 2510.974 kb on + strand, within BPHYT_RS11055at 2510.975 kb on - strand, within BPHYT_RS11055at 2511.205 kb on + strand, within BPHYT_RS11055at 2511.205 kb on + strand, within BPHYT_RS11055at 2511.248 kb on + strand, within BPHYT_RS11055at 2511.288 kb on + strandat 2511.289 kb on - strandat 2511.289 kb on - strandat 2511.419 kb on - strandat 2511.425 kb on - strandat 2511.504 kb on - strandat 2511.789 kb on + strand, within BPHYT_RS11060at 2512.750 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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2,510,854 + BPHYT_RS11055 0.36 +0.1
2,510,974 + BPHYT_RS11055 0.52 -0.7
2,510,974 + BPHYT_RS11055 0.52 +1.7
2,510,975 - BPHYT_RS11055 0.52 -0.9
2,511,205 + BPHYT_RS11055 0.82 -0.9
2,511,205 + BPHYT_RS11055 0.82 -2.9
2,511,248 + BPHYT_RS11055 0.88 +0.6
2,511,288 + +1.5
2,511,289 - +1.1
2,511,289 - +0.2
2,511,419 - +1.9
2,511,425 - -1.1
2,511,504 - +1.3
2,511,789 + BPHYT_RS11060 0.27 -1.4
2,512,750 + -0.5

Or see this region's nucleotide sequence