Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10620

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10610 and BPHYT_RS10615 are separated by 36 nucleotidesBPHYT_RS10615 and BPHYT_RS10620 are separated by 56 nucleotidesBPHYT_RS10620 and BPHYT_RS10625 are separated by 119 nucleotides BPHYT_RS10610: BPHYT_RS10610 - LysR family transcriptional regulator, at 2,418,493 to 2,419,389 _RS10610 BPHYT_RS10615: BPHYT_RS10615 - phosphonoacetaldehyde hydrolase, at 2,419,426 to 2,420,229 _RS10615 BPHYT_RS10620: BPHYT_RS10620 - 2-aminoethylphosphonate:pyruvate aminotransferase, at 2,420,286 to 2,421,518 _RS10620 BPHYT_RS10625: BPHYT_RS10625 - LysR family transcriptional regulator, at 2,421,638 to 2,422,546 _RS10625 Position (kb) 2420 2421 2422Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2419.395 kb on + strandat 2419.410 kb on + strandat 2419.411 kb on - strandat 2419.467 kb on - strandat 2419.505 kb on + strandat 2419.736 kb on + strand, within BPHYT_RS10615at 2419.746 kb on - strand, within BPHYT_RS10615at 2419.746 kb on - strand, within BPHYT_RS10615at 2421.053 kb on + strand, within BPHYT_RS10620at 2421.107 kb on - strand, within BPHYT_RS10620at 2421.129 kb on - strand, within BPHYT_RS10620at 2421.175 kb on + strand, within BPHYT_RS10620at 2421.247 kb on + strand, within BPHYT_RS10620at 2421.320 kb on + strand, within BPHYT_RS10620at 2421.561 kb on - strandat 2421.853 kb on + strand, within BPHYT_RS10625at 2422.287 kb on - strand, within BPHYT_RS10625at 2422.489 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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2,419,395 + +0.1
2,419,410 + -0.7
2,419,411 - -0.6
2,419,467 - -1.8
2,419,505 + -1.9
2,419,736 + BPHYT_RS10615 0.39 +0.3
2,419,746 - BPHYT_RS10615 0.40 +1.1
2,419,746 - BPHYT_RS10615 0.40 -0.5
2,421,053 + BPHYT_RS10620 0.62 -1.0
2,421,107 - BPHYT_RS10620 0.67 +1.9
2,421,129 - BPHYT_RS10620 0.68 +1.2
2,421,175 + BPHYT_RS10620 0.72 +0.0
2,421,247 + BPHYT_RS10620 0.78 +0.6
2,421,320 + BPHYT_RS10620 0.84 +0.7
2,421,561 - -1.5
2,421,853 + BPHYT_RS10625 0.24 -1.8
2,422,287 - BPHYT_RS10625 0.71 -0.1
2,422,489 - -3.6

Or see this region's nucleotide sequence