Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10610 and BPHYT_RS10615 are separated by 36 nucleotides BPHYT_RS10615 and BPHYT_RS10620 are separated by 56 nucleotides BPHYT_RS10620 and BPHYT_RS10625 are separated by 119 nucleotides
BPHYT_RS10610: BPHYT_RS10610 - LysR family transcriptional regulator, at 2,418,493 to 2,419,389
_RS10610
BPHYT_RS10615: BPHYT_RS10615 - phosphonoacetaldehyde hydrolase, at 2,419,426 to 2,420,229
_RS10615
BPHYT_RS10620: BPHYT_RS10620 - 2-aminoethylphosphonate:pyruvate aminotransferase, at 2,420,286 to 2,421,518
_RS10620
BPHYT_RS10625: BPHYT_RS10625 - LysR family transcriptional regulator, at 2,421,638 to 2,422,546
_RS10625
Position (kb)
2420
2421
2422 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2419.395 kb on + strand at 2419.410 kb on + strand at 2419.411 kb on - strand at 2419.467 kb on - strand at 2419.505 kb on + strand at 2419.736 kb on + strand, within BPHYT_RS10615 at 2419.746 kb on - strand, within BPHYT_RS10615 at 2419.746 kb on - strand, within BPHYT_RS10615 at 2421.053 kb on + strand, within BPHYT_RS10620 at 2421.107 kb on - strand, within BPHYT_RS10620 at 2421.129 kb on - strand, within BPHYT_RS10620 at 2421.175 kb on + strand, within BPHYT_RS10620 at 2421.247 kb on + strand, within BPHYT_RS10620 at 2421.320 kb on + strand, within BPHYT_RS10620 at 2421.561 kb on - strand at 2421.853 kb on + strand, within BPHYT_RS10625 at 2422.287 kb on - strand, within BPHYT_RS10625 at 2422.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 2,419,395 + +0.1 2,419,410 + -0.7 2,419,411 - -0.6 2,419,467 - -1.8 2,419,505 + -1.9 2,419,736 + BPHYT_RS10615 0.39 +0.3 2,419,746 - BPHYT_RS10615 0.40 +1.1 2,419,746 - BPHYT_RS10615 0.40 -0.5 2,421,053 + BPHYT_RS10620 0.62 -1.0 2,421,107 - BPHYT_RS10620 0.67 +1.9 2,421,129 - BPHYT_RS10620 0.68 +1.2 2,421,175 + BPHYT_RS10620 0.72 +0.0 2,421,247 + BPHYT_RS10620 0.78 +0.6 2,421,320 + BPHYT_RS10620 0.84 +0.7 2,421,561 - -1.5 2,421,853 + BPHYT_RS10625 0.24 -1.8 2,422,287 - BPHYT_RS10625 0.71 -0.1 2,422,489 - -3.6
Or see this region's nucleotide sequence