Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09695

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09685 and BPHYT_RS09690 are separated by 235 nucleotidesBPHYT_RS09690 and BPHYT_RS09695 are separated by 446 nucleotidesBPHYT_RS09695 and BPHYT_RS09700 are separated by 594 nucleotides BPHYT_RS09685: BPHYT_RS09685 - membrane protein, at 2,194,875 to 2,195,324 _RS09685 BPHYT_RS09690: BPHYT_RS09690 - phosphate starvation-inducible protein PsiF, at 2,195,560 to 2,195,859 _RS09690 BPHYT_RS09695: BPHYT_RS09695 - hypothetical protein, at 2,196,306 to 2,196,602 _RS09695 BPHYT_RS09700: BPHYT_RS09700 - transposase, at 2,197,197 to 2,198,372 _RS09700 Position (kb) 2196 2197Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2195.320 kb on - strandat 2195.378 kb on - strandat 2195.385 kb on + strandat 2195.385 kb on + strandat 2195.758 kb on - strand, within BPHYT_RS09690at 2195.920 kb on - strandat 2196.129 kb on + strandat 2196.229 kb on + strandat 2196.238 kb on + strandat 2196.313 kb on - strandat 2196.324 kb on + strandat 2196.324 kb on + strandat 2196.457 kb on + strand, within BPHYT_RS09695at 2196.517 kb on - strand, within BPHYT_RS09695at 2196.622 kb on - strandat 2196.698 kb on - strandat 2196.986 kb on + strandat 2197.095 kb on - strandat 2197.104 kb on - strandat 2197.213 kb on + strandat 2197.213 kb on + strandat 2197.480 kb on - strand, within BPHYT_RS09700

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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2,195,320 - -1.4
2,195,378 - +1.3
2,195,385 + -0.1
2,195,385 + -0.9
2,195,758 - BPHYT_RS09690 0.66 +1.3
2,195,920 - +0.4
2,196,129 + -0.1
2,196,229 + -0.2
2,196,238 + -0.4
2,196,313 - -0.7
2,196,324 + +0.1
2,196,324 + -0.3
2,196,457 + BPHYT_RS09695 0.51 -1.2
2,196,517 - BPHYT_RS09695 0.71 -3.4
2,196,622 - -0.8
2,196,698 - -0.2
2,196,986 + -0.3
2,197,095 - +1.1
2,197,104 - -1.1
2,197,213 + -0.2
2,197,213 + +1.8
2,197,480 - BPHYT_RS09700 0.24 +1.1

Or see this region's nucleotide sequence