Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09090 and BPHYT_RS09095 are separated by 148 nucleotides BPHYT_RS09095 and BPHYT_RS09100 are separated by 224 nucleotides BPHYT_RS09100 and BPHYT_RS09105 overlap by 4 nucleotides
BPHYT_RS09090: BPHYT_RS09090 - ABC transporter permease, at 2,056,901 to 2,057,725
_RS09090
BPHYT_RS09095: BPHYT_RS09095 - NADPH:quinone oxidoreductase, at 2,057,874 to 2,058,848
_RS09095
BPHYT_RS09100: BPHYT_RS09100 - stationary phase survival protein SurE, at 2,059,073 to 2,059,831
_RS09100
BPHYT_RS09105: BPHYT_RS09105 - protein-L-isoaspartate O-methyltransferase, at 2,059,828 to 2,060,880
_RS09105
Position (kb)
2057
2058
2059 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2057.112 kb on - strand, within BPHYT_RS09090 at 2057.235 kb on - strand, within BPHYT_RS09090 at 2057.366 kb on + strand, within BPHYT_RS09090 at 2057.494 kb on + strand, within BPHYT_RS09090 at 2057.495 kb on - strand, within BPHYT_RS09090 at 2057.537 kb on + strand, within BPHYT_RS09090 at 2057.538 kb on - strand, within BPHYT_RS09090 at 2057.602 kb on + strand, within BPHYT_RS09090 at 2057.603 kb on - strand, within BPHYT_RS09090 at 2057.603 kb on - strand, within BPHYT_RS09090 at 2058.211 kb on + strand, within BPHYT_RS09095 at 2058.277 kb on + strand, within BPHYT_RS09095 at 2058.278 kb on - strand, within BPHYT_RS09095 at 2058.698 kb on - strand, within BPHYT_RS09095 at 2058.880 kb on + strand at 2058.881 kb on - strand at 2059.010 kb on + strand at 2059.010 kb on + strand at 2059.256 kb on + strand, within BPHYT_RS09100 at 2059.256 kb on + strand, within BPHYT_RS09100 at 2059.257 kb on - strand, within BPHYT_RS09100 at 2059.315 kb on + strand, within BPHYT_RS09100 at 2059.316 kb on - strand, within BPHYT_RS09100 at 2059.316 kb on - strand, within BPHYT_RS09100
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 2,057,112 - BPHYT_RS09090 0.26 +0.8 2,057,235 - BPHYT_RS09090 0.40 -0.8 2,057,366 + BPHYT_RS09090 0.56 +1.6 2,057,494 + BPHYT_RS09090 0.72 -2.7 2,057,495 - BPHYT_RS09090 0.72 +1.6 2,057,537 + BPHYT_RS09090 0.77 +0.7 2,057,538 - BPHYT_RS09090 0.77 -0.2 2,057,602 + BPHYT_RS09090 0.85 +1.3 2,057,603 - BPHYT_RS09090 0.85 +0.6 2,057,603 - BPHYT_RS09090 0.85 -0.4 2,058,211 + BPHYT_RS09095 0.35 -0.3 2,058,277 + BPHYT_RS09095 0.41 -0.5 2,058,278 - BPHYT_RS09095 0.41 -1.5 2,058,698 - BPHYT_RS09095 0.85 -0.6 2,058,880 + -0.9 2,058,881 - +0.1 2,059,010 + +0.1 2,059,010 + +1.2 2,059,256 + BPHYT_RS09100 0.24 -0.5 2,059,256 + BPHYT_RS09100 0.24 +1.0 2,059,257 - BPHYT_RS09100 0.24 -1.0 2,059,315 + BPHYT_RS09100 0.32 -1.1 2,059,316 - BPHYT_RS09100 0.32 +4.2 2,059,316 - BPHYT_RS09100 0.32 -0.4
Or see this region's nucleotide sequence