Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08835 and BPHYT_RS08840 are separated by 306 nucleotides BPHYT_RS08840 and BPHYT_RS08845 are separated by 145 nucleotides BPHYT_RS08845 and BPHYT_RS08850 overlap by 11 nucleotides
BPHYT_RS08835: BPHYT_RS08835 - glyoxylate carboligase, at 2,001,768 to 2,003,543
_RS08835
BPHYT_RS08840: BPHYT_RS08840 - LysR family transcriptional regulator, at 2,003,850 to 2,004,764
_RS08840
BPHYT_RS08845: BPHYT_RS08845 - RNA polymerase sigma factor, at 2,004,910 to 2,005,644
_RS08845
BPHYT_RS08850: BPHYT_RS08850 - transcriptional regulator, at 2,005,634 to 2,006,569
_RS08850
Position (kb)
2003
2004
2005 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2002.941 kb on - strand, within BPHYT_RS08835 at 2002.994 kb on - strand, within BPHYT_RS08835 at 2003.011 kb on + strand, within BPHYT_RS08835 at 2003.215 kb on + strand, within BPHYT_RS08835 at 2003.256 kb on - strand, within BPHYT_RS08835 at 2003.256 kb on - strand, within BPHYT_RS08835 at 2003.281 kb on + strand, within BPHYT_RS08835 at 2003.282 kb on - strand, within BPHYT_RS08835 at 2003.282 kb on - strand, within BPHYT_RS08835 at 2003.282 kb on - strand, within BPHYT_RS08835 at 2003.327 kb on + strand, within BPHYT_RS08835 at 2003.363 kb on - strand, within BPHYT_RS08835 at 2003.363 kb on - strand, within BPHYT_RS08835 at 2003.579 kb on - strand at 2003.808 kb on + strand at 2003.808 kb on + strand at 2004.360 kb on + strand, within BPHYT_RS08840 at 2004.456 kb on + strand, within BPHYT_RS08840 at 2004.457 kb on - strand, within BPHYT_RS08840 at 2004.646 kb on - strand, within BPHYT_RS08840 at 2004.700 kb on - strand at 2004.700 kb on - strand at 2004.726 kb on + strand at 2005.013 kb on - strand, within BPHYT_RS08845 at 2005.159 kb on + strand, within BPHYT_RS08845 at 2005.642 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 2,002,941 - BPHYT_RS08835 0.66 +1.2 2,002,994 - BPHYT_RS08835 0.69 -0.1 2,003,011 + BPHYT_RS08835 0.70 +1.2 2,003,215 + BPHYT_RS08835 0.81 -1.6 2,003,256 - BPHYT_RS08835 0.84 +0.6 2,003,256 - BPHYT_RS08835 0.84 +0.6 2,003,281 + BPHYT_RS08835 0.85 -0.5 2,003,282 - BPHYT_RS08835 0.85 +1.7 2,003,282 - BPHYT_RS08835 0.85 -1.9 2,003,282 - BPHYT_RS08835 0.85 +2.2 2,003,327 + BPHYT_RS08835 0.88 +0.3 2,003,363 - BPHYT_RS08835 0.90 -0.1 2,003,363 - BPHYT_RS08835 0.90 +0.1 2,003,579 - +0.5 2,003,808 + -2.3 2,003,808 + +0.6 2,004,360 + BPHYT_RS08840 0.56 -1.3 2,004,456 + BPHYT_RS08840 0.66 -2.2 2,004,457 - BPHYT_RS08840 0.66 +0.0 2,004,646 - BPHYT_RS08840 0.87 -1.6 2,004,700 - +2.1 2,004,700 - +2.2 2,004,726 + +0.5 2,005,013 - BPHYT_RS08845 0.14 -0.4 2,005,159 + BPHYT_RS08845 0.34 +0.2 2,005,642 + -0.6
Or see this region's nucleotide sequence