Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08470 and BPHYT_RS08475 are separated by 197 nucleotides BPHYT_RS08475 and BPHYT_RS08480 overlap by 4 nucleotides BPHYT_RS08480 and BPHYT_RS08485 are separated by 207 nucleotides
BPHYT_RS08470: BPHYT_RS08470 - glycosyl transferase, at 1,937,633 to 1,938,829
_RS08470
BPHYT_RS08475: BPHYT_RS08475 - hypothetical protein, at 1,939,027 to 1,939,326
_RS08475
BPHYT_RS08480: BPHYT_RS08480 - transcriptional regulator, at 1,939,323 to 1,939,745
_RS08480
BPHYT_RS08485: BPHYT_RS08485 - undecaprenyl-phosphate glucose phosphotransferase, at 1,939,953 to 1,941,350
_RS08485
Position (kb) 1939
1940 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1938.899 kb on + strand at 1938.907 kb on + strand at 1939.139 kb on - strand, within BPHYT_RS08475 at 1939.174 kb on + strand, within BPHYT_RS08475 at 1939.174 kb on + strand, within BPHYT_RS08475 at 1939.189 kb on + strand, within BPHYT_RS08475 at 1939.243 kb on + strand, within BPHYT_RS08475 at 1939.244 kb on - strand, within BPHYT_RS08475 at 1939.275 kb on - strand, within BPHYT_RS08475 at 1939.291 kb on + strand, within BPHYT_RS08475 at 1939.426 kb on - strand, within BPHYT_RS08480 at 1939.627 kb on + strand, within BPHYT_RS08480 at 1939.892 kb on + strand at 1940.030 kb on + strand at 1940.038 kb on + strand at 1940.153 kb on + strand, within BPHYT_RS08485 at 1940.153 kb on + strand, within BPHYT_RS08485 at 1940.178 kb on + strand, within BPHYT_RS08485
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 1,938,899 + +1.0 1,938,907 + -1.3 1,939,139 - BPHYT_RS08475 0.37 +0.4 1,939,174 + BPHYT_RS08475 0.49 -1.9 1,939,174 + BPHYT_RS08475 0.49 -3.1 1,939,189 + BPHYT_RS08475 0.54 +2.0 1,939,243 + BPHYT_RS08475 0.72 +2.6 1,939,244 - BPHYT_RS08475 0.72 +0.0 1,939,275 - BPHYT_RS08475 0.83 -0.1 1,939,291 + BPHYT_RS08475 0.88 -0.4 1,939,426 - BPHYT_RS08480 0.24 +4.1 1,939,627 + BPHYT_RS08480 0.72 -0.3 1,939,892 + +1.1 1,940,030 + -0.2 1,940,038 + -0.1 1,940,153 + BPHYT_RS08485 0.14 +0.8 1,940,153 + BPHYT_RS08485 0.14 -1.9 1,940,178 + BPHYT_RS08485 0.16 +0.5
Or see this region's nucleotide sequence