Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08050 and BPHYT_RS08055 are separated by 137 nucleotides BPHYT_RS08055 and BPHYT_RS08060 are separated by 157 nucleotides BPHYT_RS08060 and BPHYT_RS08065 overlap by 4 nucleotides BPHYT_RS08065 and BPHYT_RS08070 are separated by 93 nucleotides
BPHYT_RS08050: BPHYT_RS08050 - pseudouridine synthase, at 1,835,368 to 1,837,248
_RS08050
BPHYT_RS08055: BPHYT_RS08055 - hypothetical protein, at 1,837,386 to 1,837,622
_RS08055
BPHYT_RS08060: BPHYT_RS08060 - ribosome maturation factor RimP, at 1,837,780 to 1,838,238
_RS08060
BPHYT_RS08065: BPHYT_RS08065 - transcription termination factor NusA, at 1,838,235 to 1,839,710
_RS08065
BPHYT_RS08070: BPHYT_RS08070 - translation initiation factor IF-2, at 1,839,804 to 1,842,764
_RS08070
Position (kb)
1838
1839
1840 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1837.291 kb on - strand at 1837.370 kb on + strand at 1837.371 kb on - strand at 1837.384 kb on - strand at 1837.768 kb on + strand at 1837.768 kb on + strand at 1837.768 kb on + strand at 1837.869 kb on + strand, within BPHYT_RS08060 at 1837.869 kb on + strand, within BPHYT_RS08060 at 1837.869 kb on + strand, within BPHYT_RS08060 at 1837.869 kb on + strand, within BPHYT_RS08060 at 1837.869 kb on + strand, within BPHYT_RS08060 at 1837.873 kb on + strand, within BPHYT_RS08060 at 1837.873 kb on + strand, within BPHYT_RS08060 at 1837.963 kb on + strand, within BPHYT_RS08060 at 1839.729 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 1,837,291 - -0.1 1,837,370 + -0.7 1,837,371 - +0.0 1,837,384 - -0.4 1,837,768 + +0.3 1,837,768 + +1.0 1,837,768 + +0.4 1,837,869 + BPHYT_RS08060 0.19 -0.4 1,837,869 + BPHYT_RS08060 0.19 -1.0 1,837,869 + BPHYT_RS08060 0.19 -2.8 1,837,869 + BPHYT_RS08060 0.19 -1.3 1,837,869 + BPHYT_RS08060 0.19 -0.3 1,837,873 + BPHYT_RS08060 0.20 -1.0 1,837,873 + BPHYT_RS08060 0.20 -1.1 1,837,963 + BPHYT_RS08060 0.40 -0.7 1,839,729 + -0.4
Or see this region's nucleotide sequence