Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS05455

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS05450 and BPHYT_RS05455 are separated by 347 nucleotidesBPHYT_RS05455 and BPHYT_RS05460 are separated by 96 nucleotides BPHYT_RS05450: BPHYT_RS05450 - AraC family transcriptional regulator, at 1,251,673 to 1,252,074 _RS05450 BPHYT_RS05455: BPHYT_RS05455 - AraC family transcriptional regulator, at 1,252,422 to 1,253,267 _RS05455 BPHYT_RS05460: BPHYT_RS05460 - chloroperoxidase, at 1,253,364 to 1,254,194 _RS05460 Position (kb) 1252 1253 1254Strain fitness (log2 ratio) -2 -1 0 1 2at 1251.470 kb on + strandat 1251.517 kb on + strandat 1251.610 kb on - strandat 1251.892 kb on + strand, within BPHYT_RS05450at 1252.154 kb on - strandat 1252.232 kb on + strandat 1252.635 kb on + strand, within BPHYT_RS05455at 1252.635 kb on + strand, within BPHYT_RS05455at 1252.636 kb on - strand, within BPHYT_RS05455at 1252.766 kb on + strand, within BPHYT_RS05455at 1253.338 kb on - strandat 1253.605 kb on + strand, within BPHYT_RS05460at 1253.690 kb on - strand, within BPHYT_RS05460at 1253.722 kb on + strand, within BPHYT_RS05460at 1254.185 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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1,251,470 + +2.7
1,251,517 + +0.6
1,251,610 - +0.6
1,251,892 + BPHYT_RS05450 0.54 +0.6
1,252,154 - -0.0
1,252,232 + -1.6
1,252,635 + BPHYT_RS05455 0.25 +0.9
1,252,635 + BPHYT_RS05455 0.25 -0.7
1,252,636 - BPHYT_RS05455 0.25 +1.2
1,252,766 + BPHYT_RS05455 0.41 -1.0
1,253,338 - +0.3
1,253,605 + BPHYT_RS05460 0.29 -0.2
1,253,690 - BPHYT_RS05460 0.39 -2.5
1,253,722 + BPHYT_RS05460 0.43 -1.0
1,254,185 + +1.3

Or see this region's nucleotide sequence