Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS05440

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS05435 and BPHYT_RS05440 are separated by 245 nucleotidesBPHYT_RS05440 and BPHYT_RS05445 overlap by 2 nucleotides BPHYT_RS05435: BPHYT_RS05435 - ABC transporter substrate-binding protein, at 1,249,047 to 1,250,210 _RS05435 BPHYT_RS05440: BPHYT_RS05440 - hypothetical protein, at 1,250,456 to 1,250,650 _RS05440 BPHYT_RS05445: BPHYT_RS05445 - hypothetical protein, at 1,250,649 to 1,250,831 _RS05445 Position (kb) 1250 1251Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1249.542 kb on + strand, within BPHYT_RS05435at 1249.566 kb on - strand, within BPHYT_RS05435at 1249.877 kb on + strand, within BPHYT_RS05435at 1249.993 kb on - strand, within BPHYT_RS05435at 1250.151 kb on + strandat 1250.389 kb on - strandat 1250.611 kb on + strand, within BPHYT_RS05440at 1250.611 kb on + strand, within BPHYT_RS05440at 1250.632 kb on - strandat 1250.633 kb on + strandat 1250.634 kb on - strandat 1250.872 kb on + strandat 1251.470 kb on + strandat 1251.517 kb on + strandat 1251.610 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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1,249,542 + BPHYT_RS05435 0.43 +1.8
1,249,566 - BPHYT_RS05435 0.45 -0.8
1,249,877 + BPHYT_RS05435 0.71 -0.0
1,249,993 - BPHYT_RS05435 0.81 -0.6
1,250,151 + +0.2
1,250,389 - -1.4
1,250,611 + BPHYT_RS05440 0.79 -2.8
1,250,611 + BPHYT_RS05440 0.79 -2.2
1,250,632 - +0.7
1,250,633 + -1.1
1,250,634 - -0.2
1,250,872 + -3.0
1,251,470 + +2.7
1,251,517 + +0.6
1,251,610 - +0.6

Or see this region's nucleotide sequence