Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04295 and BPHYT_RS04300 are separated by 56 nucleotides BPHYT_RS04300 and BPHYT_RS04305 are separated by 89 nucleotides
BPHYT_RS04295: BPHYT_RS04295 - ABC transporter, at 984,140 to 984,928
_RS04295
BPHYT_RS04300: BPHYT_RS04300 - family 2 glycosyl transferase, at 984,985 to 987,438
_RS04300
BPHYT_RS04305: BPHYT_RS04305 - hypothetical protein, at 987,528 to 989,003
_RS04305
Position (kb) 984
985
986
987
988 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 984.273 kb on - strand, within BPHYT_RS04295 at 984.286 kb on + strand, within BPHYT_RS04295 at 984.324 kb on + strand, within BPHYT_RS04295 at 984.371 kb on + strand, within BPHYT_RS04295 at 984.371 kb on + strand, within BPHYT_RS04295 at 984.371 kb on + strand, within BPHYT_RS04295 at 984.372 kb on - strand, within BPHYT_RS04295 at 984.372 kb on - strand, within BPHYT_RS04295 at 984.478 kb on + strand, within BPHYT_RS04295 at 984.478 kb on + strand, within BPHYT_RS04295 at 984.479 kb on - strand, within BPHYT_RS04295 at 984.623 kb on + strand, within BPHYT_RS04295 at 984.647 kb on + strand, within BPHYT_RS04295 at 984.698 kb on + strand, within BPHYT_RS04295 at 984.698 kb on + strand, within BPHYT_RS04295 at 984.699 kb on - strand, within BPHYT_RS04295 at 984.841 kb on + strand, within BPHYT_RS04295 at 984.841 kb on + strand, within BPHYT_RS04295 at 985.236 kb on + strand, within BPHYT_RS04300 at 985.236 kb on + strand, within BPHYT_RS04300 at 985.237 kb on - strand, within BPHYT_RS04300 at 985.281 kb on + strand, within BPHYT_RS04300 at 985.282 kb on - strand, within BPHYT_RS04300 at 985.282 kb on - strand, within BPHYT_RS04300 at 985.282 kb on - strand, within BPHYT_RS04300 at 985.330 kb on + strand, within BPHYT_RS04300 at 985.537 kb on + strand, within BPHYT_RS04300 at 985.537 kb on + strand, within BPHYT_RS04300 at 985.538 kb on - strand, within BPHYT_RS04300 at 985.540 kb on + strand, within BPHYT_RS04300 at 985.679 kb on + strand, within BPHYT_RS04300 at 985.679 kb on + strand, within BPHYT_RS04300 at 985.681 kb on + strand, within BPHYT_RS04300 at 985.681 kb on + strand, within BPHYT_RS04300 at 985.681 kb on + strand, within BPHYT_RS04300 at 985.682 kb on - strand, within BPHYT_RS04300 at 985.682 kb on - strand, within BPHYT_RS04300 at 985.683 kb on + strand, within BPHYT_RS04300 at 985.683 kb on + strand, within BPHYT_RS04300 at 985.683 kb on + strand, within BPHYT_RS04300 at 985.684 kb on - strand, within BPHYT_RS04300 at 985.684 kb on - strand, within BPHYT_RS04300 at 986.017 kb on + strand, within BPHYT_RS04300 at 986.017 kb on + strand, within BPHYT_RS04300 at 986.018 kb on - strand, within BPHYT_RS04300 at 986.089 kb on + strand, within BPHYT_RS04300 at 986.090 kb on - strand, within BPHYT_RS04300 at 986.200 kb on - strand, within BPHYT_RS04300 at 986.529 kb on + strand, within BPHYT_RS04300 at 986.614 kb on + strand, within BPHYT_RS04300 at 986.920 kb on + strand, within BPHYT_RS04300 at 987.035 kb on - strand, within BPHYT_RS04300 at 987.035 kb on - strand, within BPHYT_RS04300 at 987.139 kb on + strand, within BPHYT_RS04300 at 987.139 kb on + strand, within BPHYT_RS04300 at 987.139 kb on + strand, within BPHYT_RS04300 at 987.220 kb on + strand at 987.280 kb on + strand at 987.281 kb on - strand at 987.281 kb on - strand at 987.498 kb on + strand at 987.538 kb on + strand at 987.538 kb on + strand at 987.538 kb on + strand at 987.538 kb on + strand at 987.539 kb on - strand at 987.539 kb on - strand at 987.539 kb on - strand at 987.543 kb on - strand at 987.677 kb on + strand, within BPHYT_RS04305 at 987.677 kb on + strand, within BPHYT_RS04305 at 988.086 kb on + strand, within BPHYT_RS04305
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 984,273 - BPHYT_RS04295 0.17 -0.7 984,286 + BPHYT_RS04295 0.19 +0.9 984,324 + BPHYT_RS04295 0.23 +1.7 984,371 + BPHYT_RS04295 0.29 -1.1 984,371 + BPHYT_RS04295 0.29 +0.2 984,371 + BPHYT_RS04295 0.29 -2.7 984,372 - BPHYT_RS04295 0.29 +1.7 984,372 - BPHYT_RS04295 0.29 +0.6 984,478 + BPHYT_RS04295 0.43 +1.8 984,478 + BPHYT_RS04295 0.43 +0.7 984,479 - BPHYT_RS04295 0.43 +1.8 984,623 + BPHYT_RS04295 0.61 +0.3 984,647 + BPHYT_RS04295 0.64 -0.9 984,698 + BPHYT_RS04295 0.71 -0.8 984,698 + BPHYT_RS04295 0.71 -2.0 984,699 - BPHYT_RS04295 0.71 +1.2 984,841 + BPHYT_RS04295 0.89 -1.1 984,841 + BPHYT_RS04295 0.89 -2.9 985,236 + BPHYT_RS04300 0.10 +1.0 985,236 + BPHYT_RS04300 0.10 +1.2 985,237 - BPHYT_RS04300 0.10 -1.2 985,281 + BPHYT_RS04300 0.12 -0.2 985,282 - BPHYT_RS04300 0.12 +2.0 985,282 - BPHYT_RS04300 0.12 +1.2 985,282 - BPHYT_RS04300 0.12 -0.2 985,330 + BPHYT_RS04300 0.14 +1.5 985,537 + BPHYT_RS04300 0.22 +0.6 985,537 + BPHYT_RS04300 0.22 +1.7 985,538 - BPHYT_RS04300 0.23 +0.2 985,540 + BPHYT_RS04300 0.23 +2.2 985,679 + BPHYT_RS04300 0.28 +2.4 985,679 + BPHYT_RS04300 0.28 +2.5 985,681 + BPHYT_RS04300 0.28 +1.9 985,681 + BPHYT_RS04300 0.28 +3.8 985,681 + BPHYT_RS04300 0.28 +2.4 985,682 - BPHYT_RS04300 0.28 +0.2 985,682 - BPHYT_RS04300 0.28 +2.4 985,683 + BPHYT_RS04300 0.28 +2.5 985,683 + BPHYT_RS04300 0.28 +1.4 985,683 + BPHYT_RS04300 0.28 +2.7 985,684 - BPHYT_RS04300 0.28 +0.2 985,684 - BPHYT_RS04300 0.28 -0.4 986,017 + BPHYT_RS04300 0.42 +1.7 986,017 + BPHYT_RS04300 0.42 +1.7 986,018 - BPHYT_RS04300 0.42 +2.9 986,089 + BPHYT_RS04300 0.45 +0.8 986,090 - BPHYT_RS04300 0.45 +1.3 986,200 - BPHYT_RS04300 0.50 +2.2 986,529 + BPHYT_RS04300 0.63 +2.4 986,614 + BPHYT_RS04300 0.66 +1.9 986,920 + BPHYT_RS04300 0.79 -0.3 987,035 - BPHYT_RS04300 0.84 -0.2 987,035 - BPHYT_RS04300 0.84 +3.5 987,139 + BPHYT_RS04300 0.88 +1.3 987,139 + BPHYT_RS04300 0.88 -2.3 987,139 + BPHYT_RS04300 0.88 +0.8 987,220 + +1.2 987,280 + +1.7 987,281 - +0.2 987,281 - +1.4 987,498 + -0.4 987,538 + +0.3 987,538 + -0.2 987,538 + -0.4 987,538 + +1.2 987,539 - +1.7 987,539 - +0.6 987,539 - +1.4 987,543 - +2.5 987,677 + BPHYT_RS04305 0.10 -0.0 987,677 + BPHYT_RS04305 0.10 +1.7 988,086 + BPHYT_RS04305 0.38 +1.8
Or see this region's nucleotide sequence