Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02360 and BPHYT_RS02365 are separated by 71 nucleotides BPHYT_RS02365 and BPHYT_RS02370 are separated by 53 nucleotides BPHYT_RS02370 and BPHYT_RS02375 overlap by 4 nucleotides BPHYT_RS02375 and BPHYT_RS02380 are separated by 105 nucleotides
BPHYT_RS02360: BPHYT_RS02360 - bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase, at 541,189 to 541,671
_RS02360
BPHYT_RS02365: BPHYT_RS02365 - hypothetical protein, at 541,743 to 541,982
_RS02365
BPHYT_RS02370: BPHYT_RS02370 - type III pantothenate kinase, at 542,036 to 542,848
_RS02370
BPHYT_RS02375: BPHYT_RS02375 - biotin--protein ligase, at 542,845 to 543,738
_RS02375
BPHYT_RS02380: BPHYT_RS02380 - ABC transporter permease, at 543,844 to 544,977
_RS02380
Position (kb) 542
543 Strain fitness (log2 ratio) -2
-1
0
1 at 541.151 kb on + strand at 541.151 kb on + strand at 541.151 kb on + strand at 541.659 kb on + strand at 541.659 kb on + strand at 541.721 kb on - strand at 541.721 kb on - strand at 541.744 kb on + strand at 541.745 kb on - strand at 541.944 kb on - strand, within BPHYT_RS02365 at 541.959 kb on + strand at 541.960 kb on - strand at 541.960 kb on - strand at 543.750 kb on + strand at 543.750 kb on + strand at 543.805 kb on + strand at 543.806 kb on - strand at 543.806 kb on - strand at 543.817 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 541,151 + -0.4 541,151 + -1.5 541,151 + -0.6 541,659 + -0.8 541,659 + -0.5 541,721 - +0.3 541,721 - +0.4 541,744 + -0.6 541,745 - -1.1 541,944 - BPHYT_RS02365 0.84 -2.6 541,959 + -0.5 541,960 - +0.0 541,960 - -0.9 543,750 + -2.1 543,750 + -0.6 543,805 + +0.2 543,806 - +0.9 543,806 - -0.2 543,817 + -0.3
Or see this region's nucleotide sequence