Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01095 and BPHYT_RS01100 are separated by 9 nucleotides BPHYT_RS01100 and BPHYT_RS01105 overlap by 8 nucleotides
BPHYT_RS01095: BPHYT_RS01095 - hypothetical protein, at 254,041 to 254,490
_RS01095
BPHYT_RS01100: BPHYT_RS01100 - protein mobD, at 254,500 to 255,204
_RS01100
BPHYT_RS01105: BPHYT_RS01105 - hypothetical protein, at 255,197 to 255,916
_RS01105
Position (kb) 254
255 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 253.099 kb on + strand at 253.171 kb on - strand at 253.211 kb on + strand at 253.266 kb on + strand at 253.314 kb on + strand at 253.416 kb on + strand at 253.494 kb on - strand at 253.531 kb on + strand at 253.585 kb on + strand at 253.824 kb on - strand at 253.854 kb on + strand at 254.197 kb on + strand, within BPHYT_RS01095 at 254.536 kb on + strand at 254.536 kb on + strand at 254.537 kb on - strand at 254.537 kb on - strand at 254.537 kb on - strand at 254.623 kb on - strand, within BPHYT_RS01100 at 254.672 kb on + strand at 254.673 kb on - strand, within BPHYT_RS01100 at 254.740 kb on + strand, within BPHYT_RS01100 at 254.962 kb on + strand, within BPHYT_RS01100 at 254.963 kb on - strand, within BPHYT_RS01100 at 254.965 kb on + strand, within BPHYT_RS01100 at 254.966 kb on - strand, within BPHYT_RS01100 at 254.966 kb on - strand, within BPHYT_RS01100 at 254.993 kb on - strand, within BPHYT_RS01100 at 255.102 kb on - strand, within BPHYT_RS01100 at 255.166 kb on + strand at 255.167 kb on - strand at 255.167 kb on - strand at 255.167 kb on - strand at 255.371 kb on + strand, within BPHYT_RS01105 at 255.374 kb on + strand, within BPHYT_RS01105 at 255.386 kb on + strand, within BPHYT_RS01105 at 255.387 kb on - strand, within BPHYT_RS01105
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 253,099 + -0.7 253,171 - +0.4 253,211 + +0.3 253,266 + +1.7 253,314 + -1.6 253,416 + +2.2 253,494 - +0.4 253,531 + -1.2 253,585 + -0.8 253,824 - +0.1 253,854 + +1.4 254,197 + BPHYT_RS01095 0.35 -1.3 254,536 + -0.1 254,536 + +0.6 254,537 - +1.3 254,537 - +0.4 254,537 - -1.0 254,623 - BPHYT_RS01100 0.17 +0.0 254,672 + +2.7 254,673 - BPHYT_RS01100 0.25 -0.5 254,740 + BPHYT_RS01100 0.34 +0.3 254,962 + BPHYT_RS01100 0.66 -0.3 254,963 - BPHYT_RS01100 0.66 +1.2 254,965 + BPHYT_RS01100 0.66 +0.2 254,966 - BPHYT_RS01100 0.66 -0.3 254,966 - BPHYT_RS01100 0.66 -0.4 254,993 - BPHYT_RS01100 0.70 +0.1 255,102 - BPHYT_RS01100 0.85 +1.1 255,166 + -3.4 255,167 - -0.3 255,167 - -2.3 255,167 - -2.3 255,371 + BPHYT_RS01105 0.24 +0.1 255,374 + BPHYT_RS01105 0.25 -0.1 255,386 + BPHYT_RS01105 0.26 +0.0 255,387 - BPHYT_RS01105 0.26 -2.4
Or see this region's nucleotide sequence