Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS36360

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS36350 and BPHYT_RS36355 are separated by 246 nucleotidesBPHYT_RS36355 and BPHYT_RS36360 are separated by 13 nucleotidesBPHYT_RS36360 and BPHYT_RS36365 are separated by 153 nucleotidesBPHYT_RS36365 and BPHYT_RS36370 are separated by 230 nucleotides BPHYT_RS36350: BPHYT_RS36350 - hypothetical protein, at 83,145 to 84,278 _RS36350 BPHYT_RS36355: BPHYT_RS36355 - hypothetical protein, at 84,525 to 84,875 _RS36355 BPHYT_RS36360: BPHYT_RS36360 - hypothetical protein, at 84,889 to 85,194 _RS36360 BPHYT_RS36365: BPHYT_RS36365 - hypothetical protein, at 85,348 to 85,650 _RS36365 BPHYT_RS36370: BPHYT_RS36370 - integrase, at 85,881 to 87,089 _RS36370 Position (kb) 84 85 86Strain fitness (log2 ratio) -2 -1 0 1at 83.914 kb on - strand, within BPHYT_RS36350at 84.004 kb on - strand, within BPHYT_RS36350at 84.423 kb on - strandat 84.801 kb on + strand, within BPHYT_RS36355at 84.803 kb on - strand, within BPHYT_RS36355at 84.980 kb on - strand, within BPHYT_RS36360at 84.997 kb on + strand, within BPHYT_RS36360at 85.099 kb on + strand, within BPHYT_RS36360at 85.619 kb on + strand, within BPHYT_RS36365at 85.675 kb on + strandat 85.990 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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83,914 - BPHYT_RS36350 0.68 +0.9
84,004 - BPHYT_RS36350 0.76 +0.1
84,423 - +0.5
84,801 + BPHYT_RS36355 0.79 -2.1
84,803 - BPHYT_RS36355 0.79 +0.4
84,980 - BPHYT_RS36360 0.30 -2.7
84,997 + BPHYT_RS36360 0.35 -2.3
85,099 + BPHYT_RS36360 0.69 -0.6
85,619 + BPHYT_RS36365 0.89 -1.0
85,675 + +0.4
85,990 - +0.5

Or see this region's nucleotide sequence