Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35235 and BPHYT_RS35240 are separated by 57 nucleotides BPHYT_RS35240 and BPHYT_RS35245 are separated by 128 nucleotides BPHYT_RS35245 and BPHYT_RS35250 are separated by 55 nucleotides BPHYT_RS35250 and BPHYT_RS35255 are separated by 149 nucleotides
BPHYT_RS35235: BPHYT_RS35235 - amino acid transporter, at 3,508,703 to 3,509,320
_RS35235
BPHYT_RS35240: BPHYT_RS35240 - membrane protein, at 3,509,378 to 3,509,956
_RS35240
BPHYT_RS35245: BPHYT_RS35245 - hypothetical protein, at 3,510,085 to 3,510,261
_RS35245
BPHYT_RS35250: BPHYT_RS35250 - alcohol dehydrogenase, at 3,510,317 to 3,511,282
_RS35250
BPHYT_RS35255: BPHYT_RS35255 - AraC family transcriptional regulator, at 3,511,432 to 3,512,262
_RS35255
Position (kb)
3510
3511
3512 Strain fitness (log2 ratio)
-2
-1
0
1 at 3509.325 kb on + strand at 3509.628 kb on + strand, within BPHYT_RS35240 at 3509.628 kb on + strand, within BPHYT_RS35240 at 3509.628 kb on + strand, within BPHYT_RS35240 at 3509.628 kb on + strand, within BPHYT_RS35240 at 3509.730 kb on + strand, within BPHYT_RS35240 at 3509.784 kb on + strand, within BPHYT_RS35240 at 3509.785 kb on - strand, within BPHYT_RS35240 at 3509.944 kb on - strand at 3510.087 kb on - strand at 3510.129 kb on + strand, within BPHYT_RS35245 at 3510.129 kb on + strand, within BPHYT_RS35245 at 3510.210 kb on - strand, within BPHYT_RS35245 at 3510.270 kb on + strand at 3510.717 kb on + strand, within BPHYT_RS35250 at 3511.869 kb on - strand, within BPHYT_RS35255 at 3511.883 kb on + strand, within BPHYT_RS35255 at 3511.995 kb on + strand, within BPHYT_RS35255 at 3512.181 kb on + strand at 3512.199 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 3,509,325 + -2.2 3,509,628 + BPHYT_RS35240 0.43 -0.0 3,509,628 + BPHYT_RS35240 0.43 +0.1 3,509,628 + BPHYT_RS35240 0.43 +0.3 3,509,628 + BPHYT_RS35240 0.43 -1.2 3,509,730 + BPHYT_RS35240 0.61 +0.3 3,509,784 + BPHYT_RS35240 0.70 -0.2 3,509,785 - BPHYT_RS35240 0.70 -0.8 3,509,944 - -0.8 3,510,087 - +1.0 3,510,129 + BPHYT_RS35245 0.25 -0.5 3,510,129 + BPHYT_RS35245 0.25 -0.9 3,510,210 - BPHYT_RS35245 0.71 +1.0 3,510,270 + -1.5 3,510,717 + BPHYT_RS35250 0.41 -1.3 3,511,869 - BPHYT_RS35255 0.53 -0.9 3,511,883 + BPHYT_RS35255 0.54 +0.5 3,511,995 + BPHYT_RS35255 0.68 -0.4 3,512,181 + +1.4 3,512,199 - -1.5
Or see this region's nucleotide sequence