Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34045 and BPHYT_RS34050 are separated by 3 nucleotides BPHYT_RS34050 and BPHYT_RS34055 overlap by 4 nucleotides BPHYT_RS34055 and BPHYT_RS34060 overlap by 4 nucleotides BPHYT_RS34060 and BPHYT_RS34065 overlap by 1 nucleotides
BPHYT_RS34045: BPHYT_RS34045 - secretion system protein F, at 3,238,817 to 3,239,665
_RS34045
BPHYT_RS34050: BPHYT_RS34050 - secretion system protein F, at 3,239,669 to 3,240,550
_RS34050
BPHYT_RS34055: BPHYT_RS34055 - hypothetical protein, at 3,240,547 to 3,240,897
_RS34055
BPHYT_RS34060: BPHYT_RS34060 - pilus biosynthesis protein TadE, at 3,240,894 to 3,241,730
_RS34060
BPHYT_RS34065: BPHYT_RS34065 - lytic transglycosylase, at 3,241,730 to 3,242,545
_RS34065
Position (kb)
3240
3241 Strain fitness (log2 ratio)
-1
0
1 at 3239.912 kb on - strand, within BPHYT_RS34050 at 3240.056 kb on + strand, within BPHYT_RS34050 at 3240.125 kb on + strand, within BPHYT_RS34050 at 3240.126 kb on - strand, within BPHYT_RS34050 at 3240.305 kb on - strand, within BPHYT_RS34050 at 3240.566 kb on - strand at 3241.444 kb on - strand, within BPHYT_RS34060 at 3241.485 kb on + strand, within BPHYT_RS34060 at 3241.485 kb on + strand, within BPHYT_RS34060 at 3241.566 kb on + strand, within BPHYT_RS34060 at 3241.671 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 3,239,912 - BPHYT_RS34050 0.28 -0.6 3,240,056 + BPHYT_RS34050 0.44 -0.8 3,240,125 + BPHYT_RS34050 0.52 -1.1 3,240,126 - BPHYT_RS34050 0.52 -0.8 3,240,305 - BPHYT_RS34050 0.72 -0.1 3,240,566 - -0.5 3,241,444 - BPHYT_RS34060 0.66 +0.8 3,241,485 + BPHYT_RS34060 0.71 +0.7 3,241,485 + BPHYT_RS34060 0.71 +0.7 3,241,566 + BPHYT_RS34060 0.80 -0.4 3,241,671 - +0.0
Or see this region's nucleotide sequence