Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30815

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30810 and BPHYT_RS30815 are separated by 113 nucleotidesBPHYT_RS30815 and BPHYT_RS30820 are separated by 213 nucleotides BPHYT_RS30810: BPHYT_RS30810 - transcriptional regulator, at 2,468,534 to 2,469,283 _RS30810 BPHYT_RS30815: BPHYT_RS30815 - 2-methylcitrate dehydratase, at 2,469,397 to 2,470,680 _RS30815 BPHYT_RS30820: BPHYT_RS30820 - GntR family transcriptional regulator, at 2,470,894 to 2,471,634 _RS30820 Position (kb) 2469 2470 2471Strain fitness (log2 ratio) -2 -1 0 1at 2468.463 kb on + strandat 2468.533 kb on + strandat 2468.936 kb on + strand, within BPHYT_RS30810at 2469.282 kb on - strandat 2469.530 kb on - strand, within BPHYT_RS30815at 2469.530 kb on - strand, within BPHYT_RS30815at 2469.890 kb on + strand, within BPHYT_RS30815at 2469.891 kb on - strand, within BPHYT_RS30815at 2470.216 kb on + strand, within BPHYT_RS30815at 2470.217 kb on - strand, within BPHYT_RS30815at 2470.582 kb on - strandat 2471.127 kb on - strand, within BPHYT_RS30820at 2471.375 kb on + strand, within BPHYT_RS30820

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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2,468,463 + -0.4
2,468,533 + -0.2
2,468,936 + BPHYT_RS30810 0.54 -0.6
2,469,282 - -0.1
2,469,530 - BPHYT_RS30815 0.10 +1.1
2,469,530 - BPHYT_RS30815 0.10 -1.2
2,469,890 + BPHYT_RS30815 0.38 -1.8
2,469,891 - BPHYT_RS30815 0.38 +0.5
2,470,216 + BPHYT_RS30815 0.64 -1.3
2,470,217 - BPHYT_RS30815 0.64 -0.1
2,470,582 - -0.1
2,471,127 - BPHYT_RS30820 0.31 -0.3
2,471,375 + BPHYT_RS30820 0.65 -0.7

Or see this region's nucleotide sequence