Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28085 and BPHYT_RS28090 are separated by 70 nucleotides BPHYT_RS28090 and BPHYT_RS28095 are separated by 32 nucleotides BPHYT_RS28095 and BPHYT_RS28100 are separated by 25 nucleotides BPHYT_RS28100 and BPHYT_RS28105 are separated by 55 nucleotides
BPHYT_RS28085: BPHYT_RS28085 - Fis family transcriptional regulator, at 1,863,862 to 1,865,853
_RS28085
BPHYT_RS28090: BPHYT_RS28090 - ferredoxin, at 1,865,924 to 1,866,235
_RS28090
BPHYT_RS28095: BPHYT_RS28095 - membrane protein, at 1,866,268 to 1,866,462
_RS28095
BPHYT_RS28100: BPHYT_RS28100 - hypothetical protein, at 1,866,488 to 1,866,682
_RS28100
BPHYT_RS28105: BPHYT_RS28105 - hypothetical protein, at 1,866,738 to 1,867,382
_RS28105
Position (kb)
1865
1866
1867 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1865.330 kb on - strand, within BPHYT_RS28085 at 1865.330 kb on - strand, within BPHYT_RS28085 at 1865.333 kb on - strand, within BPHYT_RS28085 at 1865.812 kb on - strand at 1865.890 kb on + strand at 1865.891 kb on - strand at 1866.104 kb on + strand, within BPHYT_RS28090 at 1866.105 kb on - strand, within BPHYT_RS28090 at 1866.153 kb on - strand, within BPHYT_RS28090 at 1866.153 kb on - strand, within BPHYT_RS28090 at 1866.575 kb on - strand, within BPHYT_RS28100 at 1866.591 kb on + strand, within BPHYT_RS28100 at 1866.648 kb on + strand, within BPHYT_RS28100 at 1866.649 kb on - strand, within BPHYT_RS28100 at 1866.773 kb on - strand at 1866.811 kb on + strand, within BPHYT_RS28105 at 1866.935 kb on - strand, within BPHYT_RS28105
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 1,865,330 - BPHYT_RS28085 0.74 -0.0 1,865,330 - BPHYT_RS28085 0.74 +0.4 1,865,333 - BPHYT_RS28085 0.74 -1.2 1,865,812 - +0.1 1,865,890 + +0.4 1,865,891 - -0.5 1,866,104 + BPHYT_RS28090 0.58 +4.4 1,866,105 - BPHYT_RS28090 0.58 -0.7 1,866,153 - BPHYT_RS28090 0.73 +0.5 1,866,153 - BPHYT_RS28090 0.73 +0.9 1,866,575 - BPHYT_RS28100 0.45 -1.8 1,866,591 + BPHYT_RS28100 0.53 +0.1 1,866,648 + BPHYT_RS28100 0.82 -0.8 1,866,649 - BPHYT_RS28100 0.83 -0.8 1,866,773 - -0.2 1,866,811 + BPHYT_RS28105 0.11 -1.3 1,866,935 - BPHYT_RS28105 0.31 +1.3
Or see this region's nucleotide sequence