Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS26815

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS26805 and BPHYT_RS26810 are separated by 148 nucleotidesBPHYT_RS26810 and BPHYT_RS26815 are separated by 164 nucleotidesBPHYT_RS26815 and BPHYT_RS26820 overlap by 4 nucleotides BPHYT_RS26805: BPHYT_RS26805 - methionine sulfoxide reductase B, at 1,586,922 to 1,587,446 _RS26805 BPHYT_RS26810: BPHYT_RS26810 - AsnC family transcriptional regulator, at 1,587,595 to 1,588,122 _RS26810 BPHYT_RS26815: BPHYT_RS26815 - 1-aminocyclopropane-1-carboxylate deaminase, at 1,588,287 to 1,589,303 _RS26815 BPHYT_RS26820: BPHYT_RS26820 - LysR family transcriptional regulator, at 1,589,300 to 1,590,187 _RS26820 Position (kb) 1588 1589 1590Strain fitness (log2 ratio) -2 -1 0 1at 1587.597 kb on - strandat 1588.455 kb on + strand, within BPHYT_RS26815at 1588.753 kb on - strand, within BPHYT_RS26815at 1588.860 kb on + strand, within BPHYT_RS26815at 1589.241 kb on + strandat 1589.242 kb on - strandat 1589.277 kb on + strandat 1589.383 kb on - strandat 1589.616 kb on + strand, within BPHYT_RS26820at 1589.679 kb on + strand, within BPHYT_RS26820at 1590.139 kb on + strandat 1590.254 kb on + strandat 1590.255 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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1,587,597 - -0.7
1,588,455 + BPHYT_RS26815 0.17 -1.4
1,588,753 - BPHYT_RS26815 0.46 -0.4
1,588,860 + BPHYT_RS26815 0.56 -2.0
1,589,241 + -2.3
1,589,242 - -0.4
1,589,277 + +0.5
1,589,383 - -1.7
1,589,616 + BPHYT_RS26820 0.36 +0.5
1,589,679 + BPHYT_RS26820 0.43 +1.1
1,590,139 + -1.2
1,590,254 + -0.5
1,590,255 - +0.8

Or see this region's nucleotide sequence