Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26685 and BPHYT_RS26690 are separated by 0 nucleotides BPHYT_RS26690 and BPHYT_RS26695 overlap by 13 nucleotides BPHYT_RS26695 and BPHYT_RS26700 are separated by 15 nucleotides BPHYT_RS26700 and BPHYT_RS26705 overlap by 4 nucleotides
BPHYT_RS26685: BPHYT_RS26685 - precorrin-8X methylmutase, at 1,555,879 to 1,556,505
_RS26685
BPHYT_RS26690: BPHYT_RS26690 - precorrin-2 C20-methyltransferase, at 1,556,506 to 1,557,237
_RS26690
BPHYT_RS26695: BPHYT_RS26695 - cobalamin biosynthesis protein CbiG, at 1,557,225 to 1,558,937
_RS26695
BPHYT_RS26700: BPHYT_RS26700 - hypothetical protein, at 1,558,953 to 1,559,564
_RS26700
BPHYT_RS26705: BPHYT_RS26705 - hypothetical protein, at 1,559,561 to 1,560,043
_RS26705
Position (kb) 1557
1558
1559 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1556.313 kb on + strand, within BPHYT_RS26685 at 1556.611 kb on + strand, within BPHYT_RS26690 at 1556.854 kb on + strand, within BPHYT_RS26690 at 1556.855 kb on - strand, within BPHYT_RS26690 at 1556.860 kb on + strand, within BPHYT_RS26690 at 1556.860 kb on + strand, within BPHYT_RS26690 at 1556.860 kb on + strand, within BPHYT_RS26690 at 1558.287 kb on + strand, within BPHYT_RS26695 at 1558.287 kb on + strand, within BPHYT_RS26695 at 1558.671 kb on + strand, within BPHYT_RS26695 at 1558.672 kb on - strand, within BPHYT_RS26695 at 1558.806 kb on - strand at 1558.811 kb on - strand at 1558.998 kb on + strand at 1558.998 kb on + strand at 1559.027 kb on + strand, within BPHYT_RS26700 at 1559.056 kb on + strand, within BPHYT_RS26700 at 1559.064 kb on + strand, within BPHYT_RS26700 at 1559.145 kb on + strand, within BPHYT_RS26700 at 1559.159 kb on - strand, within BPHYT_RS26700 at 1559.183 kb on - strand, within BPHYT_RS26700 at 1559.199 kb on - strand, within BPHYT_RS26700 at 1559.492 kb on - strand, within BPHYT_RS26700 at 1559.680 kb on + strand, within BPHYT_RS26705 at 1559.681 kb on - strand, within BPHYT_RS26705 at 1559.881 kb on - strand, within BPHYT_RS26705 at 1559.883 kb on + strand, within BPHYT_RS26705 at 1559.884 kb on - strand, within BPHYT_RS26705
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 1,556,313 + BPHYT_RS26685 0.69 +2.0 1,556,611 + BPHYT_RS26690 0.14 +0.3 1,556,854 + BPHYT_RS26690 0.48 +0.6 1,556,855 - BPHYT_RS26690 0.48 -0.5 1,556,860 + BPHYT_RS26690 0.48 -0.0 1,556,860 + BPHYT_RS26690 0.48 -0.4 1,556,860 + BPHYT_RS26690 0.48 +0.1 1,558,287 + BPHYT_RS26695 0.62 -0.9 1,558,287 + BPHYT_RS26695 0.62 +2.1 1,558,671 + BPHYT_RS26695 0.84 -0.5 1,558,672 - BPHYT_RS26695 0.84 -2.0 1,558,806 - -1.5 1,558,811 - +0.4 1,558,998 + -0.7 1,558,998 + -0.7 1,559,027 + BPHYT_RS26700 0.12 -0.3 1,559,056 + BPHYT_RS26700 0.17 -0.5 1,559,064 + BPHYT_RS26700 0.18 -0.4 1,559,145 + BPHYT_RS26700 0.31 +0.4 1,559,159 - BPHYT_RS26700 0.34 -0.2 1,559,183 - BPHYT_RS26700 0.38 -1.0 1,559,199 - BPHYT_RS26700 0.40 -0.9 1,559,492 - BPHYT_RS26700 0.88 +1.5 1,559,680 + BPHYT_RS26705 0.25 +0.7 1,559,681 - BPHYT_RS26705 0.25 -1.9 1,559,881 - BPHYT_RS26705 0.66 +0.3 1,559,883 + BPHYT_RS26705 0.67 +1.0 1,559,884 - BPHYT_RS26705 0.67 +0.5
Or see this region's nucleotide sequence