Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24275 and BPHYT_RS24280 are separated by 398 nucleotides BPHYT_RS24280 and BPHYT_RS24285 are separated by 134 nucleotides BPHYT_RS24285 and BPHYT_RS24290 are separated by 92 nucleotides
BPHYT_RS24275: BPHYT_RS24275 - alpha-ketoglutarate decarboxylase, at 1,011,630 to 1,012,904
_RS24275
BPHYT_RS24280: BPHYT_RS24280 - DSBA oxidoreductase, at 1,013,303 to 1,014,844
_RS24280
BPHYT_RS24285: BPHYT_RS24285 - TetR family transcriptional regulator, at 1,014,979 to 1,015,704
_RS24285
BPHYT_RS24290: BPHYT_RS24290 - hypothetical protein, at 1,015,797 to 1,016,516
_RS24290
Position (kb) 1013
1014
1015 Strain fitness (log2 ratio) -1
0
1 at 1012.379 kb on - strand, within BPHYT_RS24275 at 1012.529 kb on - strand, within BPHYT_RS24275 at 1012.814 kb on - strand at 1013.088 kb on + strand at 1013.700 kb on + strand, within BPHYT_RS24280 at 1013.701 kb on - strand, within BPHYT_RS24280 at 1013.701 kb on - strand, within BPHYT_RS24280 at 1014.186 kb on + strand, within BPHYT_RS24280 at 1014.186 kb on + strand, within BPHYT_RS24280 at 1014.253 kb on + strand, within BPHYT_RS24280 at 1014.253 kb on + strand, within BPHYT_RS24280 at 1014.643 kb on + strand, within BPHYT_RS24280 at 1015.037 kb on + strand at 1015.222 kb on + strand, within BPHYT_RS24285 at 1015.223 kb on - strand, within BPHYT_RS24285 at 1015.764 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 1,012,379 - BPHYT_RS24275 0.59 -1.2 1,012,529 - BPHYT_RS24275 0.71 -0.7 1,012,814 - -1.6 1,013,088 + +0.7 1,013,700 + BPHYT_RS24280 0.26 +0.3 1,013,701 - BPHYT_RS24280 0.26 +1.4 1,013,701 - BPHYT_RS24280 0.26 -0.0 1,014,186 + BPHYT_RS24280 0.57 +0.6 1,014,186 + BPHYT_RS24280 0.57 +0.1 1,014,253 + BPHYT_RS24280 0.62 -0.2 1,014,253 + BPHYT_RS24280 0.62 -0.1 1,014,643 + BPHYT_RS24280 0.87 +0.5 1,015,037 + +0.6 1,015,222 + BPHYT_RS24285 0.33 -0.9 1,015,223 - BPHYT_RS24285 0.34 +0.0 1,015,764 - -0.8
Or see this region's nucleotide sequence