Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19985 and BPHYT_RS19990 are separated by 41 nucleotides BPHYT_RS19990 and BPHYT_RS19995 are separated by 205 nucleotides BPHYT_RS19995 and BPHYT_RS20000 are separated by 6 nucleotides
BPHYT_RS19985: BPHYT_RS19985 - CoA-transferase, at 26,533 to 27,732
_RS19985
BPHYT_RS19990: BPHYT_RS19990 - MFS transporter, at 27,774 to 29,207
_RS19990
BPHYT_RS19995: BPHYT_RS19995 - response regulator receiver, at 29,413 to 29,763
_RS19995
BPHYT_RS20000: BPHYT_RS20000 - serine/threonine protein kinase, at 29,770 to 31,245
_RS20000
Position (kb) 27
28
29
30 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 27.308 kb on + strand, within BPHYT_RS19985 at 27.663 kb on - strand at 27.694 kb on + strand at 27.695 kb on - strand at 28.661 kb on - strand, within BPHYT_RS19990 at 28.726 kb on + strand, within BPHYT_RS19990 at 28.726 kb on + strand, within BPHYT_RS19990 at 28.726 kb on + strand, within BPHYT_RS19990 at 28.727 kb on - strand, within BPHYT_RS19990 at 28.727 kb on - strand, within BPHYT_RS19990 at 28.991 kb on - strand, within BPHYT_RS19990 at 29.023 kb on + strand, within BPHYT_RS19990 at 29.024 kb on - strand, within BPHYT_RS19990 at 29.044 kb on + strand, within BPHYT_RS19990 at 29.045 kb on - strand, within BPHYT_RS19990 at 29.288 kb on + strand at 29.289 kb on - strand at 29.323 kb on + strand at 29.324 kb on - strand at 29.324 kb on - strand at 29.327 kb on + strand at 29.328 kb on - strand at 29.415 kb on - strand at 29.475 kb on - strand, within BPHYT_RS19995 at 29.641 kb on + strand, within BPHYT_RS19995 at 29.641 kb on + strand, within BPHYT_RS19995 at 29.642 kb on - strand, within BPHYT_RS19995 at 29.686 kb on - strand, within BPHYT_RS19995 at 29.687 kb on + strand, within BPHYT_RS19995
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 27,308 + BPHYT_RS19985 0.65 -3.0 27,663 - +0.9 27,694 + -1.1 27,695 - +0.0 28,661 - BPHYT_RS19990 0.62 +0.2 28,726 + BPHYT_RS19990 0.66 -0.3 28,726 + BPHYT_RS19990 0.66 -0.9 28,726 + BPHYT_RS19990 0.66 +1.3 28,727 - BPHYT_RS19990 0.66 -1.1 28,727 - BPHYT_RS19990 0.66 -0.8 28,991 - BPHYT_RS19990 0.85 +0.1 29,023 + BPHYT_RS19990 0.87 -0.7 29,024 - BPHYT_RS19990 0.87 -0.3 29,044 + BPHYT_RS19990 0.89 -0.3 29,045 - BPHYT_RS19990 0.89 -0.0 29,288 + -0.1 29,289 - -2.1 29,323 + +1.9 29,324 - +1.3 29,324 - -2.0 29,327 + -1.2 29,328 - +1.7 29,415 - +0.3 29,475 - BPHYT_RS19995 0.18 +1.3 29,641 + BPHYT_RS19995 0.65 -1.5 29,641 + BPHYT_RS19995 0.65 +0.1 29,642 - BPHYT_RS19995 0.65 -0.1 29,686 - BPHYT_RS19995 0.78 +0.4 29,687 + BPHYT_RS19995 0.78 -0.2
Or see this region's nucleotide sequence