Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS17525

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS17515 and BPHYT_RS17520 are separated by 7 nucleotidesBPHYT_RS17520 and BPHYT_RS17525 are separated by 118 nucleotidesBPHYT_RS17525 and BPHYT_RS17530 are separated by 43 nucleotidesBPHYT_RS17530 and BPHYT_RS17535 are separated by 160 nucleotides BPHYT_RS17515: BPHYT_RS17515 - peptide chain release factor 1, at 3,967,244 to 3,968,326 _RS17515 BPHYT_RS17520: BPHYT_RS17520 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase, at 3,968,334 to 3,969,194 _RS17520 BPHYT_RS17525: BPHYT_RS17525 - glutaredoxin, at 3,969,313 to 3,969,624 _RS17525 BPHYT_RS17530: BPHYT_RS17530 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 3,969,668 to 3,970,267 _RS17530 BPHYT_RS17535: BPHYT_RS17535 - extradiol ring-cleavage dioxygenase class III protein subunit B, at 3,970,428 to 3,971,213 _RS17535 Position (kb) 3969 3970Strain fitness (log2 ratio) -2 -1 0 1at 3968.459 kb on + strand, within BPHYT_RS17520at 3968.503 kb on - strand, within BPHYT_RS17520at 3968.758 kb on + strand, within BPHYT_RS17520at 3968.758 kb on + strand, within BPHYT_RS17520at 3968.916 kb on + strand, within BPHYT_RS17520at 3968.916 kb on + strand, within BPHYT_RS17520at 3968.917 kb on - strand, within BPHYT_RS17520at 3968.918 kb on + strand, within BPHYT_RS17520at 3969.058 kb on + strand, within BPHYT_RS17520at 3969.058 kb on + strand, within BPHYT_RS17520at 3969.058 kb on + strand, within BPHYT_RS17520at 3969.059 kb on - strand, within BPHYT_RS17520at 3969.207 kb on - strandat 3969.209 kb on + strandat 3969.210 kb on - strandat 3969.252 kb on + strandat 3969.278 kb on + strandat 3969.279 kb on - strandat 3969.291 kb on + strandat 3969.536 kb on - strand, within BPHYT_RS17525at 3969.583 kb on + strand, within BPHYT_RS17525at 3969.584 kb on - strand, within BPHYT_RS17525at 3969.734 kb on + strand, within BPHYT_RS17530at 3969.817 kb on + strand, within BPHYT_RS17530at 3969.818 kb on - strand, within BPHYT_RS17530at 3969.818 kb on - strand, within BPHYT_RS17530at 3970.090 kb on + strand, within BPHYT_RS17530at 3970.090 kb on + strand, within BPHYT_RS17530at 3970.146 kb on - strand, within BPHYT_RS17530at 3970.266 kb on - strandat 3970.266 kb on - strandat 3970.299 kb on + strandat 3970.372 kb on + strandat 3970.372 kb on + strandat 3970.372 kb on + strandat 3970.372 kb on + strandat 3970.372 kb on + strandat 3970.372 kb on + strandat 3970.372 kb on + strandat 3970.373 kb on - strandat 3970.373 kb on - strandat 3970.373 kb on - strandat 3970.373 kb on - strandat 3970.373 kb on - strandat 3970.373 kb on - strandat 3970.374 kb on + strandat 3970.375 kb on - strandat 3970.375 kb on - strandat 3970.381 kb on - strandat 3970.393 kb on + strandat 3970.393 kb on + strandat 3970.393 kb on + strandat 3970.394 kb on - strandat 3970.394 kb on - strandat 3970.439 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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3,968,459 + BPHYT_RS17520 0.15 -0.0
3,968,503 - BPHYT_RS17520 0.20 -0.6
3,968,758 + BPHYT_RS17520 0.49 -1.7
3,968,758 + BPHYT_RS17520 0.49 -0.0
3,968,916 + BPHYT_RS17520 0.68 -0.6
3,968,916 + BPHYT_RS17520 0.68 -0.6
3,968,917 - BPHYT_RS17520 0.68 -0.6
3,968,918 + BPHYT_RS17520 0.68 -0.6
3,969,058 + BPHYT_RS17520 0.84 +0.2
3,969,058 + BPHYT_RS17520 0.84 -2.3
3,969,058 + BPHYT_RS17520 0.84 +1.4
3,969,059 - BPHYT_RS17520 0.84 -0.6
3,969,207 - +0.4
3,969,209 + -0.8
3,969,210 - +1.6
3,969,252 + -0.6
3,969,278 + -0.6
3,969,279 - -0.0
3,969,291 + -0.8
3,969,536 - BPHYT_RS17525 0.71 -1.6
3,969,583 + BPHYT_RS17525 0.87 -1.0
3,969,584 - BPHYT_RS17525 0.87 -1.7
3,969,734 + BPHYT_RS17530 0.11 -0.4
3,969,817 + BPHYT_RS17530 0.25 +0.1
3,969,818 - BPHYT_RS17530 0.25 -2.3
3,969,818 - BPHYT_RS17530 0.25 -0.2
3,970,090 + BPHYT_RS17530 0.70 -0.9
3,970,090 + BPHYT_RS17530 0.70 -1.0
3,970,146 - BPHYT_RS17530 0.80 -1.4
3,970,266 - +0.3
3,970,266 - -1.9
3,970,299 + +0.3
3,970,372 + -0.4
3,970,372 + +0.4
3,970,372 + +0.3
3,970,372 + -0.6
3,970,372 + -1.1
3,970,372 + +0.4
3,970,372 + -0.9
3,970,373 - +1.0
3,970,373 - -0.4
3,970,373 - +1.6
3,970,373 - -0.0
3,970,373 - +0.3
3,970,373 - -0.6
3,970,374 + -0.2
3,970,375 - +0.4
3,970,375 - -0.5
3,970,381 - +0.1
3,970,393 + +0.5
3,970,393 + -1.4
3,970,393 + +0.6
3,970,394 - -0.4
3,970,394 - +0.6
3,970,439 - -1.5

Or see this region's nucleotide sequence